@article{34218a23fdaf4b7e92df2430893aa94c,
title = "Whole-Cell Photoenzymatic Cascades to Synthesize Long-Chain Aliphatic Amines and Esters from Renewable Fatty Acids",
abstract = "Long-chain aliphatic amines such as (S,Z)-heptadec-9-en-7-amine and 9-aminoheptadecane were synthesized from ricinoleic acid and oleic acid, respectively, by whole-cell cascade reactions using the combination of an alcohol dehydrogenase (ADH) from Micrococcus luteus, an engineered amine transaminase from Vibrio fluvialis (Vf-ATA), and a photoactivated decarboxylase from Chlorella variabilis NC64A (Cv-FAP) in a one-pot process. In addition, long chain aliphatic esters such as 10-(heptanoyloxy)dec-8-ene and octylnonanoate were prepared from ricinoleic acid and oleic acid, respectively, by using the combination of the ADH, a Baeyer–Villiger monooxygenase variant from Pseudomonas putida KT2440, and the Cv-FAP. The target compounds were produced at rates of up to 37 U g−1 dry cells with conversions up to 90 %. Therefore, this study contributes to the preparation of industrially relevant long-chain aliphatic chiral amines and esters from renewable fatty acid resources.",
keywords = "Biocatalysis, fatty acids, long-chain aliphatic amines, photodecarboxylase, whole-cell biocatalysis",
author = "Cha, {Hee Jeong} and Hwang, {Se Yeun} and Lee, {Da Som} and Kumar, {Akula Ravi} and Kwon, {Yong Uk} and Moritz Vo{\ss} and Eva Schuiten and Bornscheuer, {Uwe T.} and Frank Hollmann and Oh, {Deok Kun} and Park, {Jin Byung}",
note = "Funding Information: This work was supported by C1 Gas Refinery Research Center (NRF Grant Number: 2018M3D3A1A01055735‐3) of the National Research Foundation (NRF) of Korea funded by Ministry of Science and ICT and by the Marine Biomaterials Research Center grant from the Marine Biotechnology Program [No. D11013214H480000100] funded by the Ministry of Oceans and Fisheries, Korea. F.H. gratefully acknowledges support by The Netherlands Organisation for Scientific Research through a VICI grant (No. 724.014.003) and the European Research Commission (ERC consolidator grant No. 648026). U.T.B. gratefully acknowledges support by the European Union's Horizon 2020 Programme (Marie‐Curie Actions, grant No. 722610) and the German Research Foundation, DFG (grant No.: BO 1862/16‐1). Funding Information: This work was supported by C1 Gas Refinery Research Center (NRF Grant Number: 2018M3D3A1A01055735-3) of the National Research Foundation (NRF) of Korea funded by Ministry of Science and ICT and by the Marine Biomaterials Research Center grant from the Marine Biotechnology Program [No. D11013214H480000100] funded by the Ministry of Oceans and Fisheries, Korea. F.H. gratefully acknowledges support by The Netherlands Organisation for Scientific Research through a VICI grant (No. 724.014.003) and the European Research Commission (ERC consolidator grant No. 648026). U.T.B. gratefully acknowledges support by the European Union's Horizon 2020 Programme (Marie-Curie Actions, grant No. 722610) and the German Research Foundation, DFG (grant No.: BO 1862/16-1). Publisher Copyright: {\textcopyright} 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2020",
month = apr,
day = "27",
doi = "10.1002/anie.201915108",
language = "English",
volume = "59",
pages = "7024--7028",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
number = "18",
}