Whole-blood diagnostic sensing system based on populational platelet rolling behavior

A. J. Ricco, N. J. Kent, B. Lincoln, G. R. Meade, L. Basabe-Desmonts, L. P. Lee, B. D. MacCraith, B. G. Corcoran, D. Kenny

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We are developing a point-of-care diagnostic sensor system for early warning of diseases or disorders involving the activation state of platelets. The system tracks platelet movement - adhering, releasing, rolling - along a well-characterized protein surface using customized image analysis to objectively quantify fluid-shear-mediated platelet-surface interactions for statistically meaningful numbers of platelets from a single blood sample. The fluidic device provides well-defined shear at the surface at physiological rate (∼ 1500/s) and hydrodynamically focuses the sample stream into contact with the surface, providing assay results from < 200 μL of undiluted whole blood. Frame-by-frame object tracking of translocating platelets provides individual trajectories via a position-based probability function. Initial results show the relative fraction of time spent by platelets in the mobile relative to the stationary state is approximately 1.6 times longer for blood treated with abciximab (ReoPro) than for untreated normal blood.

Original languageEnglish
Title of host publicationECS Transactions - 35 Years of Chemical Sensors - An Honorary Symposium for Professor Jiri Janata's 70th Birthday Celebration
PublisherElectrochemical Society Inc.
Pages73-77
Number of pages5
Edition6
ISBN (Electronic)9781607680642
ISBN (Print)9781566777148
DOIs
StatePublished - 2009
Event35 Years of Chemical Sensors - An Honorary Symposium for Professor Jiri Janata's 70th Birthday Celebration - 215th ECS Meeting - San Francisco, CA, United States
Duration: 24 May 200929 May 2009

Publication series

NameECS Transactions
Number6
Volume19
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

Conference

Conference35 Years of Chemical Sensors - An Honorary Symposium for Professor Jiri Janata's 70th Birthday Celebration - 215th ECS Meeting
Country/TerritoryUnited States
CitySan Francisco, CA
Period24/05/0929/05/09

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