TY - JOUR
T1 - Vulcanisaeta thermophila sp. nov., a hyperthermophilic and acidophilic crenarchaeon isolated from solfataric soil
AU - Yim, Kyung June
AU - Cha, In Tae
AU - Rhee, Jin Kyu
AU - Song, Hye Seon
AU - Hyun, Dong Wook
AU - Lee, Hae Won
AU - Kim, Daekyung
AU - Kim, Kil Nam
AU - Nam, Young Do
AU - Seo, Myung Ji
AU - Bae, Jin Woo
AU - Roh, Seong Woon
N1 - Publisher Copyright:
© 2015 IUMS.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - An anaerobic, rod-shaped, hyperthermophilic and acidophilic crenarchaeon, designated strain CBA1501T, was isolated from solfataric soil of the Mayon volcano in the Republic of the Philippines. Phylogenetic analysis showed that strain CBA1501T is affiliated with the genus Vulcanisaeta in the phylumCrenarchaeota. DNA sequence similarities between the 16S rRNA gene of strain CBA1501T and those of Vulcanisaeta distributa IC-017T and Vulcanisaeta souniana IC-059T were 98.5 and 97.4 %, respectively. Strain CBA1501T grew between 75–90 °C, over a pH range of 4.0–6.0 and in the presence of 0–1.0 % (w/v) NaCl, with optimal growth occurring at 85 °C, pH 5.0, and with 0 % (w/v) NaCl. Fumarate, malate, oxidized glutathione, sulfur and thiosulfate were used as final electron acceptors, but FeCl3, nitrate and sulfate were not. The DNA G+C content of strain CBA1501T was 43.1 mol%. On the basis of polyphasic taxonomic analysis, strain CBA1501T represents a novel species of the genusVulcanisaeta in the phylum Crenarchaeota, for which we propose the nameVulcanisaeta thermophila sp. nov. The type strain is CBA1501T ( = ATCC BAA-2415T = JCM 17228T).
AB - An anaerobic, rod-shaped, hyperthermophilic and acidophilic crenarchaeon, designated strain CBA1501T, was isolated from solfataric soil of the Mayon volcano in the Republic of the Philippines. Phylogenetic analysis showed that strain CBA1501T is affiliated with the genus Vulcanisaeta in the phylumCrenarchaeota. DNA sequence similarities between the 16S rRNA gene of strain CBA1501T and those of Vulcanisaeta distributa IC-017T and Vulcanisaeta souniana IC-059T were 98.5 and 97.4 %, respectively. Strain CBA1501T grew between 75–90 °C, over a pH range of 4.0–6.0 and in the presence of 0–1.0 % (w/v) NaCl, with optimal growth occurring at 85 °C, pH 5.0, and with 0 % (w/v) NaCl. Fumarate, malate, oxidized glutathione, sulfur and thiosulfate were used as final electron acceptors, but FeCl3, nitrate and sulfate were not. The DNA G+C content of strain CBA1501T was 43.1 mol%. On the basis of polyphasic taxonomic analysis, strain CBA1501T represents a novel species of the genusVulcanisaeta in the phylum Crenarchaeota, for which we propose the nameVulcanisaeta thermophila sp. nov. The type strain is CBA1501T ( = ATCC BAA-2415T = JCM 17228T).
UR - http://www.scopus.com/inward/record.url?scp=84921481977&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.065862-0
DO - 10.1099/ijs.0.065862-0
M3 - Article
C2 - 25323594
AN - SCOPUS:84921481977
SN - 1466-5026
VL - 65
SP - 201
EP - 205
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 1
ER -