Visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe

Dabin Kim; Gyoungmi Kim; Sang Jip Nam; Jun Yin; Juyoung Yoon

doi:10.1038/srep08488

Visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe

Dabin Kim, Gyoungmi Kim, Sang Jip Nam, Jun Yin, Juyoung Yoon

Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H₂O₂) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H₂O₂ not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H₂O₂. These results support that the probe can function as an efficient indicator to detect H₂O₂.

Original language	English
Article number	8488
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep08488
State	Published - 2015

Bibliographical note

Funding Information:
This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814). Jun Yin also acknowledges financial support from National Natural Science Foundation of China (21402057).

Publisher Copyright:
© 2015 Macmillan Publishers Limited.

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title = "Visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe",

abstract = "Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H2O2) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H2O2 not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H2O2. These results support that the probe can function as an efficient indicator to detect H2O2.",

author = "Dabin Kim and Gyoungmi Kim and Nam, {Sang Jip} and Jun Yin and Juyoung Yoon",

note = "Funding Information: This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814). Jun Yin also acknowledges financial support from National Natural Science Foundation of China (21402057). Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited.",

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AU - Kim, Dabin

AU - Kim, Gyoungmi

AU - Nam, Sang Jip

AU - Yin, Jun

AU - Yoon, Juyoung

N1 - Funding Information: This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814). Jun Yin also acknowledges financial support from National Natural Science Foundation of China (21402057). Publisher Copyright: © 2015 Macmillan Publishers Limited.

PY - 2015

Y1 - 2015

N2 - Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H2O2) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H2O2 not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H2O2. These results support that the probe can function as an efficient indicator to detect H2O2.

AB - Reactive oxygen species (ROS) play crucial roles in diverse physiological processes; therefore, the efficient detection of ROS is very crucial. In this study, we report a boronate-based hydrogen peroxide (H2O2) probe having naphthalimide fluorophore. This probe also contained a morpholine moiety as a directing group for lysosome. The recognition property indicated that the probe exhibited high selectivity towards H2O2 not only in the solution but also in the living cells. Furthermore, it was used to monitor the level of endogenous and exogenous H2O2. These results support that the probe can function as an efficient indicator to detect H2O2.

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Visualization of endogenous and exogenous hydrogen peroxide using a lysosome-targetable fluorescent probe

Abstract

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