Abstract
While the natural carbohydrate alginate has enabled effective three-dimensional (3D) extrusion bioprinting, it still suffers from some issues such as low printability and resolution and limited cellular function due to ionic crosslinking dependency. Here, we prepared a harmless visible light-based photocrosslinkable alginate by chemically bonding tyrosine-like residues onto alginate chains to propose a new microgel manufacturing system for the development of 3D-printed bioinks. The photocrosslinkable tyramine-conjugated alginate microgel achieved both higher cell viability and printing resolution compared to the bulk gel form. This alginate-based jammed granular microgel bioink showed excellent 3D bioprinting ability with maintained structural stability. As a biocompatible material, the developed multiple cell-loaded photocrosslinkable alginate-based microgel bioink provided excellent proliferation and migration abilities of laden living cells, providing an effective strategy to construct implantable functional artificial organ structures for 3D bioprinting-based tissue engineering.
Original language | English |
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Article number | 120895 |
Journal | Carbohydrate Polymers |
Volume | 313 |
DOIs | |
State | Published - 1 Aug 2023 |
Bibliographical note
Funding Information:This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.
Funding Information:
This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060 ) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs , Republic of Korea.
Publisher Copyright:
© 2023 Elsevier Ltd
Keywords
- 3D printing bioink
- Jammed microgels
- Multiple cell-laden structures
- Photocrosslinkable alginate
- Tyramine-conjugated alginate