Visible light-crosslinkable tyramine-conjugated alginate-based microgel bioink for multiple cell-laden 3D artificial organ

Sangmin Lee; Geunho Choi; Yun Jung Yang; Kye Il Joo; Hyung Joon Cha

doi:10.1016/j.carbpol.2023.120895

Visible light-crosslinkable tyramine-conjugated alginate-based microgel bioink for multiple cell-laden 3D artificial organ

Sangmin Lee, Geunho Choi, Yun Jung Yang, Kye Il Joo, Hyung Joon Cha

Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

While the natural carbohydrate alginate has enabled effective three-dimensional (3D) extrusion bioprinting, it still suffers from some issues such as low printability and resolution and limited cellular function due to ionic crosslinking dependency. Here, we prepared a harmless visible light-based photocrosslinkable alginate by chemically bonding tyrosine-like residues onto alginate chains to propose a new microgel manufacturing system for the development of 3D-printed bioinks. The photocrosslinkable tyramine-conjugated alginate microgel achieved both higher cell viability and printing resolution compared to the bulk gel form. This alginate-based jammed granular microgel bioink showed excellent 3D bioprinting ability with maintained structural stability. As a biocompatible material, the developed multiple cell-loaded photocrosslinkable alginate-based microgel bioink provided excellent proliferation and migration abilities of laden living cells, providing an effective strategy to construct implantable functional artificial organ structures for 3D bioprinting-based tissue engineering.

Original language	English
Article number	120895
Journal	Carbohydrate Polymers
Volume	313
DOIs	https://doi.org/10.1016/j.carbpol.2023.120895
State	Published - 1 Aug 2023

Bibliographical note

Funding Information:
This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.

Funding Information:
This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060 ) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs , Republic of Korea.

Publisher Copyright:
© 2023 Elsevier Ltd

Keywords

3D printing bioink
Jammed microgels
Multiple cell-laden structures
Photocrosslinkable alginate
Tyramine-conjugated alginate

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10.1016/j.carbpol.2023.120895

Cite this

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title = "Visible light-crosslinkable tyramine-conjugated alginate-based microgel bioink for multiple cell-laden 3D artificial organ",

abstract = "While the natural carbohydrate alginate has enabled effective three-dimensional (3D) extrusion bioprinting, it still suffers from some issues such as low printability and resolution and limited cellular function due to ionic crosslinking dependency. Here, we prepared a harmless visible light-based photocrosslinkable alginate by chemically bonding tyrosine-like residues onto alginate chains to propose a new microgel manufacturing system for the development of 3D-printed bioinks. The photocrosslinkable tyramine-conjugated alginate microgel achieved both higher cell viability and printing resolution compared to the bulk gel form. This alginate-based jammed granular microgel bioink showed excellent 3D bioprinting ability with maintained structural stability. As a biocompatible material, the developed multiple cell-loaded photocrosslinkable alginate-based microgel bioink provided excellent proliferation and migration abilities of laden living cells, providing an effective strategy to construct implantable functional artificial organ structures for 3D bioprinting-based tissue engineering.",

keywords = "3D printing bioink, Jammed microgels, Multiple cell-laden structures, Photocrosslinkable alginate, Tyramine-conjugated alginate",

author = "Sangmin Lee and Geunho Choi and Yang, {Yun Jung} and Joo, {Kye Il} and Cha, {Hyung Joon}",

note = "Funding Information: This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. Funding Information: This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060 ) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs , Republic of Korea. Publisher Copyright: {\textcopyright} 2023 Elsevier Ltd",

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N1 - Funding Information: This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs, Republic of Korea. Funding Information: This work was financially supported by the Creative Innovation Program funded by the Pohang Steel Company and the High Value-added Food Technology Development Program (grant number: 321025051HD060 ) through the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) funded by the Ministry of Agriculture, Food and Rural Affairs , Republic of Korea. Publisher Copyright: © 2023 Elsevier Ltd

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N2 - While the natural carbohydrate alginate has enabled effective three-dimensional (3D) extrusion bioprinting, it still suffers from some issues such as low printability and resolution and limited cellular function due to ionic crosslinking dependency. Here, we prepared a harmless visible light-based photocrosslinkable alginate by chemically bonding tyrosine-like residues onto alginate chains to propose a new microgel manufacturing system for the development of 3D-printed bioinks. The photocrosslinkable tyramine-conjugated alginate microgel achieved both higher cell viability and printing resolution compared to the bulk gel form. This alginate-based jammed granular microgel bioink showed excellent 3D bioprinting ability with maintained structural stability. As a biocompatible material, the developed multiple cell-loaded photocrosslinkable alginate-based microgel bioink provided excellent proliferation and migration abilities of laden living cells, providing an effective strategy to construct implantable functional artificial organ structures for 3D bioprinting-based tissue engineering.

AB - While the natural carbohydrate alginate has enabled effective three-dimensional (3D) extrusion bioprinting, it still suffers from some issues such as low printability and resolution and limited cellular function due to ionic crosslinking dependency. Here, we prepared a harmless visible light-based photocrosslinkable alginate by chemically bonding tyrosine-like residues onto alginate chains to propose a new microgel manufacturing system for the development of 3D-printed bioinks. The photocrosslinkable tyramine-conjugated alginate microgel achieved both higher cell viability and printing resolution compared to the bulk gel form. This alginate-based jammed granular microgel bioink showed excellent 3D bioprinting ability with maintained structural stability. As a biocompatible material, the developed multiple cell-loaded photocrosslinkable alginate-based microgel bioink provided excellent proliferation and migration abilities of laden living cells, providing an effective strategy to construct implantable functional artificial organ structures for 3D bioprinting-based tissue engineering.

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Visible light-crosslinkable tyramine-conjugated alginate-based microgel bioink for multiple cell-laden 3D artificial organ

Abstract

Bibliographical note

Keywords

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