Visible light-crosslinkable tyramine-conjugated alginate-based microgel bioink for multiple cell-laden 3D artificial organ

Sangmin Lee, Geunho Choi, Yun Jung Yang, Kye Il Joo, Hyung Joon Cha

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

While the natural carbohydrate alginate has enabled effective three-dimensional (3D) extrusion bioprinting, it still suffers from some issues such as low printability and resolution and limited cellular function due to ionic crosslinking dependency. Here, we prepared a harmless visible light-based photocrosslinkable alginate by chemically bonding tyrosine-like residues onto alginate chains to propose a new microgel manufacturing system for the development of 3D-printed bioinks. The photocrosslinkable tyramine-conjugated alginate microgel achieved both higher cell viability and printing resolution compared to the bulk gel form. This alginate-based jammed granular microgel bioink showed excellent 3D bioprinting ability with maintained structural stability. As a biocompatible material, the developed multiple cell-loaded photocrosslinkable alginate-based microgel bioink provided excellent proliferation and migration abilities of laden living cells, providing an effective strategy to construct implantable functional artificial organ structures for 3D bioprinting-based tissue engineering.

Original languageEnglish
Article number120895
JournalCarbohydrate Polymers
Volume313
DOIs
StatePublished - 1 Aug 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Ltd

Keywords

  • 3D printing bioink
  • Jammed microgels
  • Multiple cell-laden structures
  • Photocrosslinkable alginate
  • Tyramine-conjugated alginate

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