Variable virulence of biotype 3 Vibrio vulnificus due to MARTX toxin effector domain composition

Byoung Sik Kim, Hannah E. Gavin, Karla J.F. Satchell

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Vibrio vulnificus is an environmental organism that causes septic human infections characterized by high morbidity and mortality. The annual incidence and global distribution of this pathogen are increasing as ocean waters warm. Clinical strains exhibit variations in the primary virulence toxin, suggesting a potential for the emergence of new strains with altered virulence properties. A clonal outbreak of tilapia-associated wound infections in Israel serves as a natural experiment for the sudden emergence of a new V. vulnificus strain. The effector domain content of the multifunctional autoprocessing RTX (MARTX) toxin of the outbreak-associated biotype 3 (BT3) strains was previously shown to harbor a modification generated by recombination. The modification introduced an actin-induced adenylate cyclase effector domain (ExoY) and an effector domain that disrupts the Golgi organelle (DmX). Here, we report that the exchange of these effector domains for a putative progenitor biotype 1 toxin arrangement produces a toxin that slows the lysis kinetics of targeted epithelial cells but increases cellular rounding phenotypes in response to bacteria. In addition, replacing the biotype 3 toxin variant with the putative progenitor biotype 1 variant renders the resulting strain significantly more virulent in mice. This suggests that the exchange of MARTX effector domains during the emergence of BT3 generated a toxin with reduced toxin potency, resulting in decreased virulence of this outbreak-associated strain. We posit that selection for reduced virulence may serve as a route for this lethal infectious agent to enter the human food chain by allowing it to persist in natural hosts.

Original languageEnglish
Article numbere00272-17
JournalmSphere
Volume2
Issue number4
DOIs
StatePublished - 1 Jul 2017

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (grant number 2013R1A6A3A03024337 to B.S.K.), the USDA National Institute of Food and Agriculture (a predoctoral fellowship to H.E.G.), and the National Institutes of Health (grant numbers R01AI092825 and R01AI098369 to K.J.F.S.). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication

Publisher Copyright:
© 2017 Kim et al.

Keywords

  • Biotype 3
  • Cytotoxins
  • MARTX
  • Mouse
  • Recombination
  • Vibrio vulnificus
  • Virulence factors

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