Valorization of single-carbon chemicals by using carboligases as key enzymes

Huijin Cheon, Jun Hong Kim, Jeong Sun Kim, Jin Byung Park

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations


Single-carbon (C1) biorefinery plays a key role in the consumption of global greenhouse gases and a circular carbon economy. Thereby, we have focused on the valorization of C1 compounds (e.g. methanol, formaldehyde, and formate) into multicarbon products, including bioplastic monomers, glycolate, and ethylene glycol. For instance, methanol, derived from the oxidation of CH4, can be converted into glycolate, ethylene glycol, or erythrulose via formaldehyde and glycolaldehyde, employing C1 and/or C2 carboligases as essential enzymes. Escherichia coli was engineered to convert formate, produced from CO via CO2 or from CO2 directly, into glycolate. Recent progress in the design of biotransformation pathways, enzyme discovery, and engineering, as well as whole-cell biocatalyst engineering for C1 biorefinery, was addressed in this review.

Original languageEnglish
Article number103047
JournalCurrent Opinion in Biotechnology
StatePublished - Feb 2024

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