Vacuum soft lithography to direct neuronal polarization

J. Tanner Nevill, Alexander Mo, Branden J. Cord, Theo D. Palmer, Mu Ming Poo, Luke P. Lee, Sarah C. Heilshorn

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


The ability to coat surfaces with pre-determined patterns of biomolecules by soft lithography has found use in areas ranging from fundamental biology to translational medicine, such as tissue engineering and diagnostics. However, existing surface patterning techniques (e.g., microcontact printing and traditional lithography) are unable to pattern several biomolecules in a single step. Here we introduce a simple method to simultaneously pattern multiple biomolecules in complex two-dimensional configurations onto substrates with better than 2 m resolution. This protocol, termed vacuum soft lithography, utilized below ambient pressures temporarily stored within a removable microfluidic template to expose specific regions of a substrate to multiple biochemical solutions. We demonstrate the utility of this vacuum soft lithography technique by fabricating a multi-component array that directs the adhesion, polarization, and neurite guidance of primary hippocampal neurons.

Original languageEnglish
Pages (from-to)343-347
Number of pages5
JournalSoft Matter
Issue number2
StatePublished - 21 Jan 2011


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