Vaccination with a latch peptide provides serotypeindependent protection against group B streptococcus infection in mice

Shun Mei Lin; A. Yeung Jang; Yong Zhi; Shuang Gao; Sangyong Lim; Jae Hyang Lim; Joon Young Song; Paul M. Sullam; Joon Haeng Rhee; Ho Seong Seo

doi:10.1093/infdis/jix565

Vaccination with a latch peptide provides serotypeindependent protection against group B streptococcus infection in mice

Shun Mei Lin, A. Yeung Jang, Yong Zhi, Shuang Gao, Sangyong Lim, Jae Hyang Lim, Joon Young Song, Paul M. Sullam, Joon Haeng Rhee, Ho Seong Seo

Department of Microbiology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Streptococcus agalactiae (group B streptococcus [GBS]) is a leading cause of invasive diseases in neonates and severe infections in elderly individuals. GBS serine-rich repeat glycoprotein 1 (Srr1) acts as a critical virulence factor by facilitating GBS invasion into the central nervous system through interaction with the fbrinogen Aa chain. This study revealed that srr1 is highly conserved, with 86.7% of GBS clinical isolates expressing the protein. Vaccination of mice with different Srr1 truncated peptides revealed that only Srr1 truncates containing the latch domain protected against GBS meningiThis. Furthermore, the latch peptide alone was immunogenic and elicited protective antibodies, which efciently enhanced antibody-mediated opsonophagocytic killing of GBS by HL60 cells and provided heterogeneous protection against 4 different GBS serogroups. Taken together, these fndings indicated that the latch domain of Srr1 may constitute an effective peptide vaccine candidate for GBS.

Original language	English
Pages (from-to)	93-102
Number of pages	10
Journal	Journal of Infectious Diseases
Volume	217
Issue number	1
DOIs	https://doi.org/10.1093/infdis/jix565
State	Published - 1 Jan 2018

Bibliographical note

Publisher Copyright:
© The Author(s) 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved.

Keywords

Latch
Serine-rich repeat
Streptococcus agalactiae
Vaccine

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10.1093/infdis/jix565

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title = "Vaccination with a latch peptide provides serotypeindependent protection against group B streptococcus infection in mice",

abstract = "Streptococcus agalactiae (group B streptococcus [GBS]) is a leading cause of invasive diseases in neonates and severe infections in elderly individuals. GBS serine-rich repeat glycoprotein 1 (Srr1) acts as a critical virulence factor by facilitating GBS invasion into the central nervous system through interaction with the fbrinogen Aa chain. This study revealed that srr1 is highly conserved, with 86.7% of GBS clinical isolates expressing the protein. Vaccination of mice with different Srr1 truncated peptides revealed that only Srr1 truncates containing the latch domain protected against GBS meningiThis. Furthermore, the latch peptide alone was immunogenic and elicited protective antibodies, which efciently enhanced antibody-mediated opsonophagocytic killing of GBS by HL60 cells and provided heterogeneous protection against 4 different GBS serogroups. Taken together, these fndings indicated that the latch domain of Srr1 may constitute an effective peptide vaccine candidate for GBS.",

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author = "Lin, {Shun Mei} and Jang, {A. Yeung} and Yong Zhi and Shuang Gao and Sangyong Lim and Lim, {Jae Hyang} and Song, {Joon Young} and Sullam, {Paul M.} and Rhee, {Joon Haeng} and Seo, {Ho Seong}",

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AU - Lin, Shun Mei

AU - Jang, A. Yeung

AU - Zhi, Yong

AU - Gao, Shuang

AU - Lim, Sangyong

AU - Lim, Jae Hyang

AU - Song, Joon Young

AU - Sullam, Paul M.

AU - Rhee, Joon Haeng

AU - Seo, Ho Seong

PY - 2018/1/1

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N2 - Streptococcus agalactiae (group B streptococcus [GBS]) is a leading cause of invasive diseases in neonates and severe infections in elderly individuals. GBS serine-rich repeat glycoprotein 1 (Srr1) acts as a critical virulence factor by facilitating GBS invasion into the central nervous system through interaction with the fbrinogen Aa chain. This study revealed that srr1 is highly conserved, with 86.7% of GBS clinical isolates expressing the protein. Vaccination of mice with different Srr1 truncated peptides revealed that only Srr1 truncates containing the latch domain protected against GBS meningiThis. Furthermore, the latch peptide alone was immunogenic and elicited protective antibodies, which efciently enhanced antibody-mediated opsonophagocytic killing of GBS by HL60 cells and provided heterogeneous protection against 4 different GBS serogroups. Taken together, these fndings indicated that the latch domain of Srr1 may constitute an effective peptide vaccine candidate for GBS.

AB - Streptococcus agalactiae (group B streptococcus [GBS]) is a leading cause of invasive diseases in neonates and severe infections in elderly individuals. GBS serine-rich repeat glycoprotein 1 (Srr1) acts as a critical virulence factor by facilitating GBS invasion into the central nervous system through interaction with the fbrinogen Aa chain. This study revealed that srr1 is highly conserved, with 86.7% of GBS clinical isolates expressing the protein. Vaccination of mice with different Srr1 truncated peptides revealed that only Srr1 truncates containing the latch domain protected against GBS meningiThis. Furthermore, the latch peptide alone was immunogenic and elicited protective antibodies, which efciently enhanced antibody-mediated opsonophagocytic killing of GBS by HL60 cells and provided heterogeneous protection against 4 different GBS serogroups. Taken together, these fndings indicated that the latch domain of Srr1 may constitute an effective peptide vaccine candidate for GBS.

KW - Latch

KW - Serine-rich repeat

KW - Streptococcus agalactiae

KW - Vaccine

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JO - Journal of Infectious Diseases

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Vaccination with a latch peptide provides serotypeindependent protection against group B streptococcus infection in mice

Abstract

Bibliographical note

Keywords

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