TY - JOUR
T1 - Utilization of swine wastewater as a feedstock for the production of polyhydroxyalkanoates by Azotobacter vinelandii UWD
AU - Cho, Kyung Suk
AU - Wook Ryu, Hee
AU - Park, Chang Ho
AU - Goodrich, Philip R.
N1 - Funding Information:
This work was supportedb y Kyung Hee University Research Fund (year 2QOOF ree Competition Program). The authors also wish to acknowledgeth e financials upporto f Korea ResearchF oun-dation provided in the program year of 1998( project 1998-001-E01383).
PY - 2001
Y1 - 2001
N2 - Azotobacter vinelandii UWD produced 0.69 g·l-1 poly(hydroxybutyrate-co-hydroxyvalerate, PHBV) with 7.9 mol% hydroxyvalerate (HV) from twofold-diluted swine wastewater (SW). When supplemented with 20 g·l-1 glucose, twofold-diluted SW increased copolymer production by 8.6 times. When three organic acids (acetate, propionate and butyrate) present in SW were supplemented with 20 g·l-1 glucose, PHBV production was comparable (5.5 g·l-1) to that in the case of using twofold-diluted SW supplemented with 20 g·l-1 glucose. However, the HV level (1.1-1.3 mol%) was very low. The component in SW contributing to copolymer production was found to be valerate. By 20 mM valerate 0.2 g·l-1 PHBV with 44.6 mol% HV was produced. With 30 g·l-1 glucose supplementation, 4.0 g·l-1 PHBV with 22 mol% HV was produced. The optimal ratios of carbon to phosphorus (C:P) and to nitrogen (C:N) were 165:1 and 22:1, respectively.
AB - Azotobacter vinelandii UWD produced 0.69 g·l-1 poly(hydroxybutyrate-co-hydroxyvalerate, PHBV) with 7.9 mol% hydroxyvalerate (HV) from twofold-diluted swine wastewater (SW). When supplemented with 20 g·l-1 glucose, twofold-diluted SW increased copolymer production by 8.6 times. When three organic acids (acetate, propionate and butyrate) present in SW were supplemented with 20 g·l-1 glucose, PHBV production was comparable (5.5 g·l-1) to that in the case of using twofold-diluted SW supplemented with 20 g·l-1 glucose. However, the HV level (1.1-1.3 mol%) was very low. The component in SW contributing to copolymer production was found to be valerate. By 20 mM valerate 0.2 g·l-1 PHBV with 44.6 mol% HV was produced. With 30 g·l-1 glucose supplementation, 4.0 g·l-1 PHBV with 22 mol% HV was produced. The optimal ratios of carbon to phosphorus (C:P) and to nitrogen (C:N) were 165:1 and 22:1, respectively.
KW - Azotobacter vinelandii UWD
KW - Biodegradable plastics
KW - Polyhydroxyalkanoates
KW - Swine wastewater
UR - http://www.scopus.com/inward/record.url?scp=0035101334&partnerID=8YFLogxK
U2 - 10.1016/S1389-1723(01)80054-2
DO - 10.1016/S1389-1723(01)80054-2
M3 - Article
C2 - 16232963
AN - SCOPUS:0035101334
SN - 1389-1723
VL - 91
SP - 129
EP - 133
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 2
ER -