TY - JOUR
T1 - Utilization of noxious weed water hyacinth biomass as a potential feedstock for biopolymers production
T2 - A novel approach
AU - Saratale, Rijuta Ganesh
AU - Cho, Si Kyung
AU - Ghodake, Gajanan S.
AU - Shin, Han Seung
AU - Saratale, Ganesh Dattatraya
AU - Park, Yooheon
AU - Lee, Hee Seok
AU - Bharagava, Ram Naresh
AU - Kim, Dong Su
N1 - Funding Information:
This research was supported by the collaborative R&BD program (2016~2018) of Agency for Korea National Food Cluster (AnFC). This research was supported by the collaborative R&BD program (2016~2018) of Agency for Korea National Food Cluster (AnFC).
Publisher Copyright:
© 2020 by the authors.
PY - 2020/8
Y1 - 2020/8
N2 - This study aims to utilize a noxiousweedwater hyacinth biomass (WH) for polyhydroxybutyrate (PHB) production. Alkaline and peracetic acid pretreatment was employed for the hydrolysis of WH and consequently enzymatic saccharification to produce fermentable sugars for PHB production. The pretreatment competence was determined using various operational parameters. By applying ambient conditions, alkaline pretreatment gave higher lignin removal of 65.0%, with 80.8% hydrolysis yield, and on enzyme hydrolysis (40 FPU/g of dry WH), produced total reducing sugar of about 523 mg/g of WH. The resulted WH enzymatic hydolysates were evaluated for the production of PHB by Ralstonia eutropha (ATCC 17699). The WH hydrolysates cultivation was compared to synthetic hydrolysates that contain a similar carbon composition in terms of bacterial growth and PHB synthesis. The effects of various supplements to enhance PHB production were estimated. Supplementation of corn steep liquor (CSL) as a cheap nitrogen source with WH hydrolysates favored a higher amount of PHB synthesis (73%), PHB titer of 7.30 g/L and PHB yield of 0.429 g/g of reducing sugar. Finally, using standard analytical tools, the physical and thermal characteristics of the extracted PHB were evaluated. The findings revealed WH was a promising and technically feasible option for transforming biomass into sustainable biopolymer conversion on a large scale.
AB - This study aims to utilize a noxiousweedwater hyacinth biomass (WH) for polyhydroxybutyrate (PHB) production. Alkaline and peracetic acid pretreatment was employed for the hydrolysis of WH and consequently enzymatic saccharification to produce fermentable sugars for PHB production. The pretreatment competence was determined using various operational parameters. By applying ambient conditions, alkaline pretreatment gave higher lignin removal of 65.0%, with 80.8% hydrolysis yield, and on enzyme hydrolysis (40 FPU/g of dry WH), produced total reducing sugar of about 523 mg/g of WH. The resulted WH enzymatic hydolysates were evaluated for the production of PHB by Ralstonia eutropha (ATCC 17699). The WH hydrolysates cultivation was compared to synthetic hydrolysates that contain a similar carbon composition in terms of bacterial growth and PHB synthesis. The effects of various supplements to enhance PHB production were estimated. Supplementation of corn steep liquor (CSL) as a cheap nitrogen source with WH hydrolysates favored a higher amount of PHB synthesis (73%), PHB titer of 7.30 g/L and PHB yield of 0.429 g/g of reducing sugar. Finally, using standard analytical tools, the physical and thermal characteristics of the extracted PHB were evaluated. The findings revealed WH was a promising and technically feasible option for transforming biomass into sustainable biopolymer conversion on a large scale.
KW - Alkaline pretreatment
KW - Enzymatic hydrolysis
KW - Peracetic acid pretreatment
KW - Poly(3-hydroxybutyrate)
KW - Ralstonia eutropha
KW - Water hyacinth biomass
UR - http://www.scopus.com/inward/record.url?scp=85089536574&partnerID=8YFLogxK
U2 - 10.3390/POLYM12081704
DO - 10.3390/POLYM12081704
M3 - Article
AN - SCOPUS:85089536574
SN - 2073-4360
VL - 12
JO - Polymers
JF - Polymers
IS - 8
M1 - 1704
ER -