Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manila clams Ruditapes philippinarum

Kyung Il Park, Joong Ki Park, Jehee Lee, Kwang Sik Choi

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Perkinsus is the pathogen responsible for mass mortality of the Manila clam Ruditapes philippinarum. Perkinsus sp. isolated from Manila clams collected in Korean waters was assayed by polymerase chain reaction (PCR) to determine its phylogenetic affinity with other congeneric species. Regions of rRNA of Perkinsus sp. isolated from clam haemolymph were cloned and sequenced. Sequences of a non-transcribed spacer (NTS), internal transcribed spacers (ITS 1, 2) and 5.8S rRNA genes were compared to those available from other Perkinsus species. The NTS sequence of Korean Perkinsus was approximately 99.9 to 100 % similar to that of P. atlanticus and 98.06 to 98.15 % and 73.05 to 73.14 % similar to those of P. olseni and P. marinus, respectively. The ITS 1, 5.8S rRNA and ITS 2 sequences of Korean Perkinsus showed 100 % similarity to P. atlanticus and Perkinsus sp. reported from Japan. The ITS-5.8S rRNA sequences of Korean Perkinsus were 99.86 and 93.73 % similar to those of P. olseni and P. marinus, respectively. The sporulation pattern and morphology of the Korean Perkinsus were very similar to those of P. atlanticus. Our data suggest that the Perkinsus sp. isolated from clams in Korean waters is P. atlanticus, which is currently synonymous with P. olseni reported from Australia. By considering that P. olseni has taxonomic priority, Korean Perkinsus sp. is accepted as P. olseni (atlanticus).

Original languageEnglish
Pages (from-to)255-263
Number of pages9
JournalDiseases of Aquatic Organisms
Volume66
Issue number3
DOIs
StatePublished - 23 Sep 2005

Keywords

  • 5.8S rRNA
  • ITS
  • Korea
  • NTS
  • Perkinsus olseni (atlanticus)
  • Ruditapes philippinarum
  • Taxonomy

Fingerprint

Dive into the research topics of 'Use of molecular markers for species identification of Korean Perkinsus sp. isolated from Manila clams Ruditapes philippinarum'. Together they form a unique fingerprint.

Cite this