Up-Regulation of ninjurin Expression in Human Hepatocellular Carcinoma Associated with Cirrhosis and Chronic Viral Hepatitis

Jae Wha Kim, Ae Ran Moon, Joo Heon Kim, Sun Young Yoon, Goo Taeg Oh, Yong Kyung Choe, In Seong Choe

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The cDNA clones, which were highly expressed in liver tissues of hepatocellular carcinoma (HCC) patients, were identified using dot hybridization and reconfirmed by Northern blot analysis. One of the clones, ninjurin (nerve injury induced protein), showed a much higher expression level in the liver tissue of HCC patients than in normal liver tissue. Interestingly, the presence of ninjurin mRNA transcripts was detected with high intensity in HCC tissues when combined with viral infection and cirrhosis, but not with a normal liver or HCC tissue unrelated with viral infection and cirrhosis. We produced a N-terminal part of recombinant ninjurin protein, as well as a monoclonal antibody specific to this polypeptide. The intensity of immunohistochemical staining of the liver tumor tissue, and regenerating tissue for the ninjurin protein, was stronger than that of normal liver tissue. These results suggest that ninjurin may play an important role in the development of HCC combined with cirrhosis and viral infection.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalMolecules and Cells
Volume11
Issue number2
StatePublished - 30 Apr 2001

Keywords

  • Chronic Hepatitis
  • Cirrhotic Liver Tissues
  • Hepatocellular Carcinoma
  • Liver Regeneration
  • Up-Regulation of ninjurin Expression

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