Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model

Yujung Kang, Chan Park, Daham Kim, Chu Myong Seong, Kihwan Kwon, Chulhee Choi

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

We examined the protective effect of unsorted human adipose tissue-derived stem cells (hADSCs) with a short-term culture in endothelial differentiation medium on tissue repair after ischemic injury. hADSCs were isolated from human subcutaneous adipose tissue and cultured in vitro in endothelial differentiation medium for 2. wks before transplantation. Cultured hADSCs showed a typical mesenchymal stromal cell-like phenotype, positive for endothelial-specific markers including VE-cadherin, Flt-1, eNOS, and vWF but not CD31. Two hours after ligation of the femoral artery and vein, mice were injected with the unselected hADSCs locally near the surgery site and tested for tissue perfusion and repair. Tissue perfusion rates of the ischemic limbs were significantly higher in the group treated with hADSCs compared with those of the control mice as early as post-operative day 3 (median 195.3%/min; interquartile range, 82.0-321.1 vs. median 47.1%/min; interquartile range, 18.0-58.7; p= 0.001 by Friedman two-way analysis). Subsequently, the mice treated with hADSC showed better prognosis at 4. wks after surgery, and the histological analysis revealed increased vascular density and reduced muscle atrophy in the hADSC-transplanted limbs. Moreover, hADSC-treated muscle contained differentiated myocytes positive for human NF-ΚB and myogenin antigen. These results collectively indicate that unsorted hADSCs after a 2-wk-in vitro culture have a therapeutic potential in ischemic tissue injury via inducing both angiogenesis and myogenesis.

Original languageEnglish
Pages (from-to)310-316
Number of pages7
JournalMicrovascular Research
Volume80
Issue number3
DOIs
StatePublished - Dec 2010

Keywords

  • Angiogenesis
  • Cell differentiation
  • Endothelial cell
  • Image-based flow modeling
  • Perfusion
  • Peripheral vascular diseases
  • Stem cells

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