Hydrogen polysulfide (H2Sn) is an emerging topic of research because it plays a critical role in the pathological and physiological activities of an organism. Therefore, visual monitoring of H2Sn activity in living cells is critical. In our work, a two-photon fluorescent probe NI-H2S2 based on a naphthalimide skeleton was developed as a molecular tool for detecting hydrogen polysulfides in solution and live HeLa cells. NI-H2S2 showed rapid response and high sensitivity. Notably, hydrogen polysulfides in hippocampal tissue of mice could also be tracked using the probe under a two-photon fluorescence microscope. Therefore, this probe NI-H2S2 is expected to serve as a pragmatic tool for studying the physiological roles of hydrogen polysulfides in living cells and in vivo.
Bibliographical noteFunding Information:
This study was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (No. 2012R1A3A2048814 for J. Y. and 2019R1A2B5B03100278 for H.M.K). High-resolution mass spectrometer analysis was performed on the Synapt G2-HDMS mass spectrometer (Waters, Manchester, U.K.), which was operated on the MassLynx 4.1 software at KBSI (Korea Basic Science Institue, Ochang, Center of Research Equipment).
© 2020 Elsevier B.V.
- Cell imaging
- Fluorescent probe
- Hydrogen polysulfides