Two-photon fluorescence sensors for imaging NMDA receptors and monitoring release of Zn2+ from the presynaptic terminal

Xiaoqiang Chen, Chang Su Lim, Dayoung Lee, Songyi Lee, Sang Jun Park, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Synaptic Zn2+ plays an important role in neurotransmission and a neuromodulator. The development of the imaging tools for monitoring spatiotemporal changes taking place in synaptic Zn2+ concentrations is necessary in order to understand the role of Zn2+ in the function of many aspects of the glutamate system. In this work, two-photon probes 1 and 2, bearing ifenprodil-like tails that have affinity for NMDA receptors of neuronal cells, were designed and prepared. The two-photon fluorescent probe 1, which bears (N-(6-acetylnaphthalen-2-yl)-N-methylglycine) as two-photon fluorophore, enables high resolution imaging of neuronal cells. The two-photon fluorescent probe 2, which contains the di-2-picolylamine (DPA) as a Zn2+-binding site, the naphthalimide unit as the two-photon fluorophore, and the ifenprodil-like tail as the NMDA receptor binding moiety, can be employed for selective detection of Zn2+ located near the NMDA receptor and for monitoring concentration changes of Zn2+ in live neurons and hippocampal tissues.

Original languageEnglish
Pages (from-to)770-779
Number of pages10
JournalBiosensors and Bioelectronics
Volume91
DOIs
StatePublished - 15 May 2017

Keywords

  • Fluorescent probes
  • NMDA receptors
  • Presynaptic terminal
  • Two-photon imaging
  • Zinc ions

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