Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues

Liyan Chen, Myoung Ki Cho, Di Wu, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The abnormal location or generation of superoxide radical anion (O2 •-) are implicated in many diseases, including cancers; thus, development of an efficient method to detect O2 •- is of great importance. Inspired by the fluorophore-governed selective manner to O2 •- and peroxynitrite (ONOO-) of previously reported phosphinate-based fluorescence probes, in this contribution, a phosphinothioate-containing probe, TPP, was designed. The probe exhibited easy accessibility through a one-step sequence and good photostability and biocompatibility. Interestingly, TPP showed high specificity and sensitivity to O2 •- over other reactive oxygen species/nitrogen species including ONOO-. Furthermore, with the assistance of two-photon microscopy, TPP was successfully applied for imaging endogenous O2 •- in live cells and tissues.

Original languageEnglish
JournalAnalytical Chemistry
DOIs
StatePublished - 2019

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