Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues

Liyan Chen; Myoung Ki Cho; Di Wu; Hwan Myung Kim; Juyoung Yoon

doi:10.1021/acs.analchem.9b03937

Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues

Liyan Chen, Myoung Ki Cho, Di Wu, Hwan Myung Kim, Juyoung Yoon

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

The abnormal location or generation of superoxide radical anion (O₂ ^•-) are implicated in many diseases, including cancers; thus, development of an efficient method to detect O₂ ^•- is of great importance. Inspired by the fluorophore-governed selective manner to O₂ ^•- and peroxynitrite (ONOO^-) of previously reported phosphinate-based fluorescence probes, in this contribution, a phosphinothioate-containing probe, TPP, was designed. The probe exhibited easy accessibility through a one-step sequence and good photostability and biocompatibility. Interestingly, TPP showed high specificity and sensitivity to O₂ ^•- over other reactive oxygen species/nitrogen species including ONOO^-. Furthermore, with the assistance of two-photon microscopy, TPP was successfully applied for imaging endogenous O₂ ^•- in live cells and tissues.

Original language	English
Journal	Analytical Chemistry
DOIs	https://doi.org/10.1021/acs.analchem.9b03937
State	Published - 2019

Bibliographical note

Funding Information:
This study was supported financially by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A3A2048814 for J. Yoon and No. 2019R1A2B5B03100278 for H. M. Kim). The Korea Basic Science Institute (Western Seoul) is acknowledged for the LC/MS data. FAB mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high-resolution mass spectrometer.

Publisher Copyright:
© 2019 American Chemical Society.

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title = "Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues",

abstract = "The abnormal location or generation of superoxide radical anion (O2 •-) are implicated in many diseases, including cancers; thus, development of an efficient method to detect O2 •- is of great importance. Inspired by the fluorophore-governed selective manner to O2 •- and peroxynitrite (ONOO-) of previously reported phosphinate-based fluorescence probes, in this contribution, a phosphinothioate-containing probe, TPP, was designed. The probe exhibited easy accessibility through a one-step sequence and good photostability and biocompatibility. Interestingly, TPP showed high specificity and sensitivity to O2 •- over other reactive oxygen species/nitrogen species including ONOO-. Furthermore, with the assistance of two-photon microscopy, TPP was successfully applied for imaging endogenous O2 •- in live cells and tissues.",

author = "Liyan Chen and Cho, {Myoung Ki} and Di Wu and Kim, {Hwan Myung} and Juyoung Yoon",

note = "Funding Information: This study was supported financially by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A3A2048814 for J. Yoon and No. 2019R1A2B5B03100278 for H. M. Kim). The Korea Basic Science Institute (Western Seoul) is acknowledged for the LC/MS data. FAB mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high-resolution mass spectrometer. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

year = "2019",

doi = "10.1021/acs.analchem.9b03937",

language = "English",

journal = "Analytical Chemistry",

issn = "0003-2700",

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TY - JOUR

T1 - Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues

AU - Chen, Liyan

AU - Cho, Myoung Ki

AU - Wu, Di

AU - Kim, Hwan Myung

AU - Yoon, Juyoung

N1 - Funding Information: This study was supported financially by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A3A2048814 for J. Yoon and No. 2019R1A2B5B03100278 for H. M. Kim). The Korea Basic Science Institute (Western Seoul) is acknowledged for the LC/MS data. FAB mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high-resolution mass spectrometer. Publisher Copyright: © 2019 American Chemical Society.

PY - 2019

Y1 - 2019

N2 - The abnormal location or generation of superoxide radical anion (O2 •-) are implicated in many diseases, including cancers; thus, development of an efficient method to detect O2 •- is of great importance. Inspired by the fluorophore-governed selective manner to O2 •- and peroxynitrite (ONOO-) of previously reported phosphinate-based fluorescence probes, in this contribution, a phosphinothioate-containing probe, TPP, was designed. The probe exhibited easy accessibility through a one-step sequence and good photostability and biocompatibility. Interestingly, TPP showed high specificity and sensitivity to O2 •- over other reactive oxygen species/nitrogen species including ONOO-. Furthermore, with the assistance of two-photon microscopy, TPP was successfully applied for imaging endogenous O2 •- in live cells and tissues.

AB - The abnormal location or generation of superoxide radical anion (O2 •-) are implicated in many diseases, including cancers; thus, development of an efficient method to detect O2 •- is of great importance. Inspired by the fluorophore-governed selective manner to O2 •- and peroxynitrite (ONOO-) of previously reported phosphinate-based fluorescence probes, in this contribution, a phosphinothioate-containing probe, TPP, was designed. The probe exhibited easy accessibility through a one-step sequence and good photostability and biocompatibility. Interestingly, TPP showed high specificity and sensitivity to O2 •- over other reactive oxygen species/nitrogen species including ONOO-. Furthermore, with the assistance of two-photon microscopy, TPP was successfully applied for imaging endogenous O2 •- in live cells and tissues.

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DO - 10.1021/acs.analchem.9b03937

M3 - Article

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AN - SCOPUS:85074897113

SN - 0003-2700

JO - Analytical Chemistry

JF - Analytical Chemistry

ER -

Two-Photon Fluorescence Probe for Selective Monitoring of Superoxide in Live Cells and Tissues

Abstract

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