Two-photon ESIPT-based fluorescent probe using 4-hydroxyisoindoline-1,3-dione for the detection of peroxynitrite

Luling Wu, Xue Tian, Dong Joon Lee, Juyoung Yoon, Chang Su Lim, Hwan Myung Kim, Tony D. James

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Excited-state intramolecular proton transfer (ESIPT)-based fluorophores with two-photon excitation fluorescence (TPEF) are rare. Our aim with this research was to develop ESIPT-based fluorophores exhibiting TPEF. Herein, we used 4-hydroxyisoindoline-1,3-dione as a scaffold to develop a two-photon fluorescent probe BHID-Bpin, for the detection of peroxynitrite (ONOO-). BHID-Bpin exhibits excellent selectivity, sensitivity, and fast response towards ONOO- in PBS buffer solution (10 mM, pH = 7.40). Additionally, BHID-Bpin displays high photo-stability under two-photon irradiation at 750 nm. Furthermore, the probe can image endogenous ONOO- in HeLa cells and exogenous ONOO- in rat hippocampal slices at a depth of 110 μm.

Original languageEnglish
Pages (from-to)11084-11087
Number of pages4
JournalChemical Communications
Volume57
Issue number84
DOIs
StatePublished - 28 Oct 2021

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