Troglitazone enhances the apoptotic response of DLD-1 colon cancer cells to photodynamic therapy

Hyunju Park; Si Hwan Ko; Jae Myun Lee; Jeon Han Park; Youn Hee Choi

doi:10.3349/ymj.2016.57.6.1494

Troglitazone enhances the apoptotic response of DLD-1 colon cancer cells to photodynamic therapy

Hyunju Park, Si Hwan Ko, Jae Myun Lee, Jeon Han Park, Youn Hee Choi

Department of Physiology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Purpose: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. Materials and Methods: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. Results: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. Conclusion: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.

Original language	English
Pages (from-to)	1494-1499
Number of pages	6
Journal	Yonsei Medical Journal
Volume	57
Issue number	6
DOIs	https://doi.org/10.3349/ymj.2016.57.6.1494
State	Published - Nov 2016

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant funded by Korean Government (MSIP) Grant 2012R1A5A2A32671866.

Publisher Copyright:
© Yonsei University College of Medicine 2016.

Keywords

Apoptosis
PDT
Troglitazone

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@article{450a399f74ac4296842e7053fff399a0,

title = "Troglitazone enhances the apoptotic response of DLD-1 colon cancer cells to photodynamic therapy",

abstract = "Purpose: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. Materials and Methods: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. Results: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. Conclusion: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.",

keywords = "Apoptosis, PDT, Troglitazone",

author = "Hyunju Park and Ko, {Si Hwan} and Lee, {Jae Myun} and Park, {Jeon Han} and Choi, {Youn Hee}",

note = "Funding Information: This work was supported by a National Research Foundation of Korea (NRF) grant funded by Korean Government (MSIP) Grant 2012R1A5A2A32671866. Publisher Copyright: {\textcopyright} Yonsei University College of Medicine 2016.",

year = "2016",

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doi = "10.3349/ymj.2016.57.6.1494",

language = "English",

volume = "57",

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AU - Park, Hyunju

AU - Ko, Si Hwan

AU - Lee, Jae Myun

AU - Park, Jeon Han

AU - Choi, Youn Hee

N1 - Funding Information: This work was supported by a National Research Foundation of Korea (NRF) grant funded by Korean Government (MSIP) Grant 2012R1A5A2A32671866. Publisher Copyright: © Yonsei University College of Medicine 2016.

PY - 2016/11

Y1 - 2016/11

N2 - Purpose: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. Materials and Methods: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. Results: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. Conclusion: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.

AB - Purpose: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. Materials and Methods: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. Results: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. Conclusion: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.

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KW - PDT

KW - Troglitazone

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Troglitazone enhances the apoptotic response of DLD-1 colon cancer cells to photodynamic therapy

Abstract

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Keywords

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