Trichostatin A enhances proliferation and migration of vascular smooth muscle cells by downregulating thioredoxin 1

Seungjeong Song, Sang Won Kang, Chulhee Choi

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

AimsA reduction in the level of thioredoxin 1 (Trx1) has been proposed as a possible mechanism for the tumor-specific growth arrest caused by inhibition of histone deacetylases (HDACs). In this study, we investigated the effect of trichostatin A (TSA), a potent HDAC inhibitor, on the proliferation and migration of vascular smooth muscle cells (VSMCs), and we examined the role of reduced Trx1 levels in this effect.Methods and resultsTSA treatment time-dependently decreased Trx1 expression in rat VSMCs at both the mRNA and protein levels. It also enhanced platelet-derived growth factor (PDGF)-induced proliferation and migration of the VSMCs. By potentiating Akt phosphorylation, the siRNA-induced downregulation of Trx1 also enhanced VSMC proliferation and migration in response to PDGF or serum treatment. Consistent with these results, TSA administration increased neointimal thickening in a murine model of post-angioplastic restenosis.ConclusionThese data demonstrate that TSA enhances vascular proliferative activity by downregulating Trx1, thus activating an Akt-dependent pathway. Our results indicate that, in addition to its apoptotic effects in tumour cells, the downregulation of Trx1 has a proliferative role in primary VSMCs.

Original languageEnglish
Pages (from-to)241-249
Number of pages9
JournalCardiovascular Research
Volume85
Issue number1
DOIs
StatePublished - Jan 2010

Keywords

  • Histone deacetylases
  • Migration
  • Proliferation
  • Thioredoxin
  • Vascular smooth muscle cells

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