Transforming growth factor-β1 bioassay involving matrix metalloproteinase-2 induction

Gi Hyun Kim; So Young Kang; Ho Jung Kwak; Kyung Min Baek; Seung Hwa Hong; Hwa Jeong Lee; Yeo Won Sohn

doi:10.1089/jir.2009.0058

Transforming growth factor-β1 bioassay involving matrix metalloproteinase-2 induction

Gi Hyun Kim, So Young Kang, Ho Jung Kwak, Kyung Min Baek, Seung Hwa Hong, Hwa Jeong Lee, Yeo Won Sohn

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The transforming growth factor-β1 (TGF-β1) bioassay developed in this study monitors increased luciferase activity in MCF10A cells containing the matrix metalloproteinase-2 (MMP-2) promoter with a luciferase reporter and treated with increasing TGF-β1 concentrations. The response was linear in the concentration range from 75 to 2,500pg/mL. The abilities of 3 types of TGF-β in inducing MMP-2 were different. The luciferase activity induced by TGF-β1 was about 2 times more than that by TGF-β2 and TGF-β3. The MMP-2 promoter bioassay showed greater reproducibility (coefficient of variation [CV] 10%) than the previously developed anticell proliferation assay of TF-1 cell (CV 16%) and the MMP-2 zymogram assay (CV 40%). Mary Ann Liebert, Inc.

Original language	English
Pages (from-to)	667-672
Number of pages	6
Journal	Journal of Interferon and Cytokine Research
Volume	30
Issue number	9
DOIs	https://doi.org/10.1089/jir.2009.0058
State	Published - 1 Sep 2010

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title = "Transforming growth factor-β1 bioassay involving matrix metalloproteinase-2 induction",

abstract = "The transforming growth factor-β1 (TGF-β1) bioassay developed in this study monitors increased luciferase activity in MCF10A cells containing the matrix metalloproteinase-2 (MMP-2) promoter with a luciferase reporter and treated with increasing TGF-β1 concentrations. The response was linear in the concentration range from 75 to 2,500pg/mL. The abilities of 3 types of TGF-β in inducing MMP-2 were different. The luciferase activity induced by TGF-β1 was about 2 times more than that by TGF-β2 and TGF-β3. The MMP-2 promoter bioassay showed greater reproducibility (coefficient of variation [CV] 10%) than the previously developed anticell proliferation assay of TF-1 cell (CV 16%) and the MMP-2 zymogram assay (CV 40%). Mary Ann Liebert, Inc.",

author = "Kim, {Gi Hyun} and Kang, {So Young} and Kwak, {Ho Jung} and Baek, {Kyung Min} and Hong, {Seung Hwa} and Lee, {Hwa Jeong} and Sohn, {Yeo Won}",

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AU - Kim, Gi Hyun

AU - Kang, So Young

AU - Kwak, Ho Jung

AU - Baek, Kyung Min

AU - Hong, Seung Hwa

AU - Lee, Hwa Jeong

AU - Sohn, Yeo Won

PY - 2010/9/1

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AB - The transforming growth factor-β1 (TGF-β1) bioassay developed in this study monitors increased luciferase activity in MCF10A cells containing the matrix metalloproteinase-2 (MMP-2) promoter with a luciferase reporter and treated with increasing TGF-β1 concentrations. The response was linear in the concentration range from 75 to 2,500pg/mL. The abilities of 3 types of TGF-β in inducing MMP-2 were different. The luciferase activity induced by TGF-β1 was about 2 times more than that by TGF-β2 and TGF-β3. The MMP-2 promoter bioassay showed greater reproducibility (coefficient of variation [CV] 10%) than the previously developed anticell proliferation assay of TF-1 cell (CV 16%) and the MMP-2 zymogram assay (CV 40%). Mary Ann Liebert, Inc.

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Transforming growth factor-β1 bioassay involving matrix metalloproteinase-2 induction

Abstract

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