Transcriptomes of lineage-specific Drosophila neuroblasts profiled by genetic targeting and robotic sorting

Ching Po Yang, Chi Cheng Fu, Ken Sugino, Zhiyong Liu, Qingzhong Ren, Ling Yu Liu, Xiaohao Yao, Luke P. Lee, Tzumin Lee

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast produces a specific neuronal lineage. To unravel the transcriptional networks that underlie the development of distinct neuroblast lineages, we marked and isolated lineage-specific neuroblasts for RNA sequencing. We labeled particular neuroblasts throughout neurogenesis by activating a conditional neuroblast driver in specific lineages using various intersection strategies. The targeted neuroblasts were efficiently recovered using a custom-built device for robotic single-cell picking. Transcriptome analysis of mushroom body, antennal lobe and type II neuroblasts compared with non-selective neuroblasts, neurons and glia revealed a rich repertoire of transcription factors expressed among neuroblasts in diverse patterns. Besides transcription factors that are likely to be pan-neuroblast, many transcription factors exist that are selectively enriched or repressed in certain neuroblasts. The unique combinations of transcription factors present in different neuroblasts may govern the diverse lineage-specific neuron fates.

Original languageEnglish
Pages (from-to)411-421
Number of pages11
JournalDevelopment
Volume143
Issue number3
DOIs
StatePublished - 1 Feb 2016

Bibliographical note

Publisher Copyright:
© 2016. Published by The Company of Biologists Ltd.

Keywords

  • Antennal lobe
  • Mushroom body
  • Neural stem cell
  • RNA-seq
  • Transcription factor
  • Type II neuroblast

Fingerprint

Dive into the research topics of 'Transcriptomes of lineage-specific Drosophila neuroblasts profiled by genetic targeting and robotic sorting'. Together they form a unique fingerprint.

Cite this