Transcription-related element gene expression pattern differs between microglia and macrophages during inflammation

Hyung Tae Lee, Se Kye Kim, Sun Hwa Kim, Kyung Kim, Chae Hyun Lim, Jihwan Park, Tae Young Roh, Namshin Kim, Young Gyu Chai

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Objective and design: Microglia and macrophages play an important role in the innate and adaptive immune systems. Although the resident location of these cells is different, their functions during the polarization response due to various stimuli are very similar. The present study aimed to analyze differences in microglial and macrophage gene expression during inflammation. Methods: Mouse microglial BV-2 cells were exposed to LPS (10 ng/ml). The levels of gene expression were measured using real-time RT-PCR and whole transcriptome shotgun sequencing. Results: The level of Jmjd3 gene expression in activated microglia showed a similar pattern to that of macrophages. In both cell types, genes associated with the inflammation response were generally increased whereas genes associated with metabolic and biosynthetic processes were decreased. However, the expression of transcription-related elements other than genes encoding histone modification enzymes showed a significantly different pattern between microglia and macrophages. Conclusion: Although the function and the gene expression levels of histone modification enzymes showed a similar pattern in microglia and macrophages during inflammation, the expression of transcription-related elements in both cell types showed a completely different pattern.

Original languageEnglish
Pages (from-to)389-397
Number of pages9
JournalInflammation Research
Issue number5
StatePublished - May 2014

Bibliographical note

Funding Information:
Acknowledgments We are grateful to Hee-Sun Kim for providing BV-2 cells. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2011-0030049).


  • Inflammation
  • Macrophages
  • Microglia
  • RNA-seq
  • Transcription-related elements


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