Transcranial direct current stimulation combined with amantadine in repetitive mild traumatic brain injury in rats

Soo Jeong Han; Gahee Park; Jee Hyun Suh

doi:10.1186/s12868-022-00763-3

Transcranial direct current stimulation combined with amantadine in repetitive mild traumatic brain injury in rats

Soo Jeong Han, Gahee Park, Jee Hyun Suh

Department of Rehabilitation Medicine

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Background: Balance and memory deficits are common in patients with repetitive mild traumatic brain injury (mTBI). Objective: To investigate the combined effects of amantadine and transcranial direct current stimulation (tDCS) on balance and memory in repetitive mTBI rat models. Methods: In this prospective animal study, 40 repetitive mTBI rats were randomly assigned to four groups: tDCS, amantadine, combination of amantadine and anodal tDCS, and control. The tDCS group received four sessions of anodal tDCS for four consecutive days. The amantadine group received four intraperitoneal injections of amantadine for four consecutive days. The combination group received four intraperitoneal injections of amantadine and anodal tDCS for four consecutive days. Motor-evoked potential (MEP), rotarod test, and novel object test results were evaluated before mTBI, before treatment, and after treatment. Results: All groups showed significant improvements in the rotarod and novel object tests, particularly the combination group. The combination group showed a significant improvements in duration (p < 0.01) and maximal speed in the rotarod test (p < 0.01), as well as an improvement in novel object ratio (p = 0.05) and MEP amplitude (p = 0.05) after treatment. The combination group exhibited a significant increase in novel object ratio compared to the tDCS group (p = 0.04). The GFAP integral intensity of the left motor cortex and hippocampus was the lowest in the combination group. Conclusion: Combination treatment with amantadine and tDCS had positive effects on balance and memory recovery after repetitive mTBI in rats. Therefore, we expect that the combination of amantadine and tDCS may be a treatment option for patients with repetitive mTBIs.

Original language	English
Article number	76
Journal	BMC Neuroscience
Volume	23
Issue number	1
DOIs	https://doi.org/10.1186/s12868-022-00763-3
State	Published - Dec 2022

Bibliographical note

Funding Information:
This study was supported and funded by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2059711). The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2022, The Author(s).

Keywords

Balance
Memory
Mild traumatic brain injury
tDCS

Access to Document

10.1186/s12868-022-00763-3

Cite this

@article{7d8296d9b1a04cfa8d1922744e94a0f4,

title = "Transcranial direct current stimulation combined with amantadine in repetitive mild traumatic brain injury in rats",

abstract = "Background: Balance and memory deficits are common in patients with repetitive mild traumatic brain injury (mTBI). Objective: To investigate the combined effects of amantadine and transcranial direct current stimulation (tDCS) on balance and memory in repetitive mTBI rat models. Methods: In this prospective animal study, 40 repetitive mTBI rats were randomly assigned to four groups: tDCS, amantadine, combination of amantadine and anodal tDCS, and control. The tDCS group received four sessions of anodal tDCS for four consecutive days. The amantadine group received four intraperitoneal injections of amantadine for four consecutive days. The combination group received four intraperitoneal injections of amantadine and anodal tDCS for four consecutive days. Motor-evoked potential (MEP), rotarod test, and novel object test results were evaluated before mTBI, before treatment, and after treatment. Results: All groups showed significant improvements in the rotarod and novel object tests, particularly the combination group. The combination group showed a significant improvements in duration (p < 0.01) and maximal speed in the rotarod test (p < 0.01), as well as an improvement in novel object ratio (p = 0.05) and MEP amplitude (p = 0.05) after treatment. The combination group exhibited a significant increase in novel object ratio compared to the tDCS group (p = 0.04). The GFAP integral intensity of the left motor cortex and hippocampus was the lowest in the combination group. Conclusion: Combination treatment with amantadine and tDCS had positive effects on balance and memory recovery after repetitive mTBI in rats. Therefore, we expect that the combination of amantadine and tDCS may be a treatment option for patients with repetitive mTBIs.",

keywords = "Balance, Memory, Mild traumatic brain injury, tDCS",

author = "Han, {Soo Jeong} and Gahee Park and Suh, {Jee Hyun}",

note = "Funding Information: This study was supported and funded by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2059711). The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = dec,

doi = "10.1186/s12868-022-00763-3",

language = "English",

volume = "23",

journal = "BMC Neuroscience",

issn = "1471-2202",

publisher = "BioMed Central Ltd.",

number = "1",

}

TY - JOUR

T1 - Transcranial direct current stimulation combined with amantadine in repetitive mild traumatic brain injury in rats

AU - Han, Soo Jeong

AU - Park, Gahee

AU - Suh, Jee Hyun

N1 - Funding Information: This study was supported and funded by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2059711). The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: © 2022, The Author(s).

PY - 2022/12

Y1 - 2022/12

N2 - Background: Balance and memory deficits are common in patients with repetitive mild traumatic brain injury (mTBI). Objective: To investigate the combined effects of amantadine and transcranial direct current stimulation (tDCS) on balance and memory in repetitive mTBI rat models. Methods: In this prospective animal study, 40 repetitive mTBI rats were randomly assigned to four groups: tDCS, amantadine, combination of amantadine and anodal tDCS, and control. The tDCS group received four sessions of anodal tDCS for four consecutive days. The amantadine group received four intraperitoneal injections of amantadine for four consecutive days. The combination group received four intraperitoneal injections of amantadine and anodal tDCS for four consecutive days. Motor-evoked potential (MEP), rotarod test, and novel object test results were evaluated before mTBI, before treatment, and after treatment. Results: All groups showed significant improvements in the rotarod and novel object tests, particularly the combination group. The combination group showed a significant improvements in duration (p < 0.01) and maximal speed in the rotarod test (p < 0.01), as well as an improvement in novel object ratio (p = 0.05) and MEP amplitude (p = 0.05) after treatment. The combination group exhibited a significant increase in novel object ratio compared to the tDCS group (p = 0.04). The GFAP integral intensity of the left motor cortex and hippocampus was the lowest in the combination group. Conclusion: Combination treatment with amantadine and tDCS had positive effects on balance and memory recovery after repetitive mTBI in rats. Therefore, we expect that the combination of amantadine and tDCS may be a treatment option for patients with repetitive mTBIs.

AB - Background: Balance and memory deficits are common in patients with repetitive mild traumatic brain injury (mTBI). Objective: To investigate the combined effects of amantadine and transcranial direct current stimulation (tDCS) on balance and memory in repetitive mTBI rat models. Methods: In this prospective animal study, 40 repetitive mTBI rats were randomly assigned to four groups: tDCS, amantadine, combination of amantadine and anodal tDCS, and control. The tDCS group received four sessions of anodal tDCS for four consecutive days. The amantadine group received four intraperitoneal injections of amantadine for four consecutive days. The combination group received four intraperitoneal injections of amantadine and anodal tDCS for four consecutive days. Motor-evoked potential (MEP), rotarod test, and novel object test results were evaluated before mTBI, before treatment, and after treatment. Results: All groups showed significant improvements in the rotarod and novel object tests, particularly the combination group. The combination group showed a significant improvements in duration (p < 0.01) and maximal speed in the rotarod test (p < 0.01), as well as an improvement in novel object ratio (p = 0.05) and MEP amplitude (p = 0.05) after treatment. The combination group exhibited a significant increase in novel object ratio compared to the tDCS group (p = 0.04). The GFAP integral intensity of the left motor cortex and hippocampus was the lowest in the combination group. Conclusion: Combination treatment with amantadine and tDCS had positive effects on balance and memory recovery after repetitive mTBI in rats. Therefore, we expect that the combination of amantadine and tDCS may be a treatment option for patients with repetitive mTBIs.

KW - Balance

KW - Memory

KW - Mild traumatic brain injury

KW - tDCS

UR - http://www.scopus.com/inward/record.url?scp=85143675026&partnerID=8YFLogxK

U2 - 10.1186/s12868-022-00763-3

DO - 10.1186/s12868-022-00763-3

M3 - Article

C2 - 36503366

AN - SCOPUS:85143675026

SN - 1471-2202

VL - 23

JO - BMC Neuroscience

JF - BMC Neuroscience

IS - 1

M1 - 76

ER -

Transcranial direct current stimulation combined with amantadine in repetitive mild traumatic brain injury in rats

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this