Toxicoproteomic analysis of bovine aortic endothelial cell under exposure to cigarette smoking extracts

Jiho Min, Ji Young Ahn, Simranjeet Singh Sekhon, Yoon Mi Jin, Choi Kyu Park, Hyun Ju Um, Inho Jo, Yang Hoon Kim

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The major health implications of exposure to cigarette smoke that leads to disease development are still being explored. The overall protein expression pattern of bovine aortic endothelial cell (BAEC) has been studied during the treatment with cigarette smoke extract (CSE) by using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Out of an average 950 spots in silver-stained gels, the synthetic levels of 4 proteins were significantly increased, whereas 7 proteins were decreased. The identified proteins have been found to be involved in anti-inflammation (Apolipoprotein A1), energy metabolism (ATP synthase subunit d), signaling pathway (Acetyl-CoA carboxylase; GTP-binding protein), oxidative stress response (Phosphatidylinositol transfer protein beta isoform; phosphatidylinositol transfer protein alpha isoform; Vascular endothelial growth factor), carcinogenesis (Insulin-like growth factor-binding protein 2; Visinin-like protein-1), exocytosis machinery (Synaptosomal-associated protein 25) and immune response (C4b-binding protein). A significantly different protein expression pattern between the control and the CSE-exposed BAEC has been observed and demonstrated on the synthesis level analysis. The systematic approach to the analysis of proteomic responses used as well as the detailed analysis results has been useful in understanding the novel molecular mechanisms for cigarette smoke induced pathological changes. This will help in designing the systemic strategies for the development of new therapeutic approaches to smokerelated diseases.

Original languageEnglish
Pages (from-to)341-349
Number of pages9
JournalMolecular and Cellular Toxicology
Issue number4
StatePublished - Dec 2013


  • Bovine Aortic Eendothelial Cells (BAEC)
  • Cigarette smoke
  • Comparative proteomics
  • Two-dimensional electrophoresis


Dive into the research topics of 'Toxicoproteomic analysis of bovine aortic endothelial cell under exposure to cigarette smoking extracts'. Together they form a unique fingerprint.

Cite this