TY - JOUR
T1 - Therapeutic effects and immunomodulation of suanbo mineral water therapy in a murine model of atopic dermatitis
AU - Choi, Yoon Jung
AU - Lee, Hye Jin
AU - Lee, Do Hyun
AU - Woo, So Youn
AU - Lee, Kyung Ho
AU - Yun, Seong Taek
AU - Kim, Jong Moon
AU - Kim, Hong Jig
AU - Kim, Jin Wou
PY - 2013/11
Y1 - 2013/11
N2 - Background: Balneotherapy is widely used as an alternative treatment modality for AD. Although the clinical benefit of some mineral waters has been established, their mechanisms of action in alleviating AD are only partly understood. Objective: The clinical modification and immunomodulatory or anti- inflammatory effects of mineral water from the Suanbo hot springs on the differentiation and cytokine production of Th1, Th2, and regulatory T cells (Treg) were investigated using spleen, skin tissue, and serum from NC/Nga mice. Methods: The therapeutic effects of bathing in mineral water in a Dermatophagoides farinae body extract ointment (Dfb ointment)-induced AD mouse model were assessed by measuring the modified Scoring atopic dermatitis (SCORAD) index scores, transepidermal water loss (TEWL), histological and immunohistochemical changes of the skin lesion, serum levels of interferon (IFN)-γ, interleukin (IL)-4, IL-5 and immunoglobulin E, mRNA expression of IFN-γ, IL-4 and IL-5 of dorsal skin, and helper T cell differentiation in the spleen. Results: Bathing in mineral water significantly reduced the modified SCORAD index scores, TEWL, epidermal hyperplasia, and inflammatory cell infiltration. IL-4 production and Th2 cell differentiation showed a decreasing tendency with mineral water bathing, but the Th1 cells did not. On the contrary, differentiation to Treg cells was promoted with mineral water bathing. Conclusion: Balneotherapy not only has anti-inflammatory activity, but also shows positive effects on cutaneous barrier homeostasis. These results suggest that the favorable effects of balneotherapy may be mediated by modifying the Th2 response, and possibly in part by inducing Treg cell differentiation. (Ann Dermatol 25(4) 462-470, 2013).
AB - Background: Balneotherapy is widely used as an alternative treatment modality for AD. Although the clinical benefit of some mineral waters has been established, their mechanisms of action in alleviating AD are only partly understood. Objective: The clinical modification and immunomodulatory or anti- inflammatory effects of mineral water from the Suanbo hot springs on the differentiation and cytokine production of Th1, Th2, and regulatory T cells (Treg) were investigated using spleen, skin tissue, and serum from NC/Nga mice. Methods: The therapeutic effects of bathing in mineral water in a Dermatophagoides farinae body extract ointment (Dfb ointment)-induced AD mouse model were assessed by measuring the modified Scoring atopic dermatitis (SCORAD) index scores, transepidermal water loss (TEWL), histological and immunohistochemical changes of the skin lesion, serum levels of interferon (IFN)-γ, interleukin (IL)-4, IL-5 and immunoglobulin E, mRNA expression of IFN-γ, IL-4 and IL-5 of dorsal skin, and helper T cell differentiation in the spleen. Results: Bathing in mineral water significantly reduced the modified SCORAD index scores, TEWL, epidermal hyperplasia, and inflammatory cell infiltration. IL-4 production and Th2 cell differentiation showed a decreasing tendency with mineral water bathing, but the Th1 cells did not. On the contrary, differentiation to Treg cells was promoted with mineral water bathing. Conclusion: Balneotherapy not only has anti-inflammatory activity, but also shows positive effects on cutaneous barrier homeostasis. These results suggest that the favorable effects of balneotherapy may be mediated by modifying the Th2 response, and possibly in part by inducing Treg cell differentiation. (Ann Dermatol 25(4) 462-470, 2013).
KW - Atopic dermatitis
KW - Balneotherapy
KW - Mineral waters
KW - NC/NGa mice
UR - http://www.scopus.com/inward/record.url?scp=84892160889&partnerID=8YFLogxK
U2 - 10.5021/ad.2013.25.4.462
DO - 10.5021/ad.2013.25.4.462
M3 - Article
AN - SCOPUS:84892160889
SN - 1013-9087
VL - 25
SP - 462
EP - 470
JO - Annals of Dermatology
JF - Annals of Dermatology
IS - 4
ER -