Therapeutic antibodies by phage display

Research output: Contribution to journalReview articlepeer-review

26 Scopus citations


Background: Antibody phage display is a major technological platform for the generation of fully human antibodies for therapeutic purposes. The in vitro binder selection by phage display allows researchers to have more extensive control over binding parameters and facilitates the isolation of clinical candidate antibodies with desired binding and/or functional profiles. Methods: Since the invention of antibody phage display in late 1980s, significant technological advancements in the design, construction, and selection of the antibody libraries have been made, and several fully human antibodies generated by phage display are currently approved or in various clinical development stages. Results: In this review, the background and details of antibody phage display technology, and representative antibody libraries with natural or synthetic sequence diversity and different construction strategies are described. The generation, optimization, functional and biophysical properties, and preclinical and clinical developments of some of the phage display-derived therapeutic antibodies approved for use in patients or in late-stage clinical trials are also discussed. Conclusion: With evolving novel disease targets and therapeutic strategies, antibody phage display is expected to continue to play a central role in the development of the next generation of therapeutic antibodies.

Original languageEnglish
Pages (from-to)6538-6559
Number of pages22
JournalCurrent Pharmaceutical Design
Issue number43
StatePublished - 1 Dec 2016

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant for Medicinal Bioconvergence Research Center (NRF-2013M3A6A4044991) and the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2015M3A9B6029138).

Publisher Copyright:
© 2016 Bentham Science Publishers.


  • Antibodies
  • Antibody libraries
  • Fully human antibodies
  • Natural antibody libraries
  • Phage display
  • Semi-synthetic antibody libraries
  • Synthetic antibody libraries
  • Therapeutic antibodies


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