The temporal alteration of GAD67/GAD65 ratio in the gerbil hippocampal complex following seizure

Tae Cheon Kang; Hyun Sook Kim; Myung Oak Seo; Soo Young Choi; Oh Shin Kwon; Nam In Baek; Hyeon Yong Lee; Moo Ho Won

doi:10.1016/S0006-8993(01)03056-6

The temporal alteration of GAD67/GAD65 ratio in the gerbil hippocampal complex following seizure

Tae Cheon Kang, Hyun Sook Kim, Myung Oak Seo, Soo Young Choi, Oh Shin Kwon, Nam In Baek, Hyeon Yong Lee, Moo Ho Won

Department of Life Science

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

In the present study, the distribution of glutamic acid decarboxylase (GAD) isoforms in the hippocampus of the Mongolian gerbil and its association with different sequelae of spontaneous seizure were investigated to identify the roles of balance of GAD isoforms in the epileptogenesis and the recovery mechanisms in these animals. The GAD67/GAD65 ratio in the hippocampus of pre-seizure seizure sensitive (SS) gerbil was ∼3.5-fold higher as compared to seizure resistant (SR) gerbil. Following seizure, this ratio shifted to the level of SR gerbils up to 12 h postical. Therefore, the mismatched GAD67/GAD65 ratio (imbalance of GAD isoform expressions) in the hippocampus of SS gerbil implies that GABAergic neurons may be highly activated in order to regulate the increased neuronal excitability. In addition, the alteration in this ratio after seizure may be the compensatory response for reduction of epileptic activity in this animal.

Original language	English
Pages (from-to)	159-169
Number of pages	11
Journal	Brain Research
Volume	920
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(01)03056-6
State	Published - 30 Nov 2001

Bibliographical note

Funding Information:
The GAD65 (GAD-6) antibody developed by Dr D.I. Gottlieb was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242, USA. The authors would like to thank Suek Han and Kyung Jin Lee for their technical help in this study. This work was supported by grant No. 2000-1-21300-001-2 from the Basic Research Program of the Korea Science and Engineering Foundation.

Keywords

Dentate gyrus
Epilepsy
GAD65
GAD67
Gerbil
Hippocampus
Subiculum

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title = "The temporal alteration of GAD67/GAD65 ratio in the gerbil hippocampal complex following seizure",

abstract = "In the present study, the distribution of glutamic acid decarboxylase (GAD) isoforms in the hippocampus of the Mongolian gerbil and its association with different sequelae of spontaneous seizure were investigated to identify the roles of balance of GAD isoforms in the epileptogenesis and the recovery mechanisms in these animals. The GAD67/GAD65 ratio in the hippocampus of pre-seizure seizure sensitive (SS) gerbil was ∼3.5-fold higher as compared to seizure resistant (SR) gerbil. Following seizure, this ratio shifted to the level of SR gerbils up to 12 h postical. Therefore, the mismatched GAD67/GAD65 ratio (imbalance of GAD isoform expressions) in the hippocampus of SS gerbil implies that GABAergic neurons may be highly activated in order to regulate the increased neuronal excitability. In addition, the alteration in this ratio after seizure may be the compensatory response for reduction of epileptic activity in this animal.",

keywords = "Dentate gyrus, Epilepsy, GAD65, GAD67, Gerbil, Hippocampus, Subiculum",

author = "Kang, {Tae Cheon} and Kim, {Hyun Sook} and Seo, {Myung Oak} and Choi, {Soo Young} and Kwon, {Oh Shin} and Baek, {Nam In} and Lee, {Hyeon Yong} and Won, {Moo Ho}",

note = "Funding Information: The GAD65 (GAD-6) antibody developed by Dr D.I. Gottlieb was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242, USA. The authors would like to thank Suek Han and Kyung Jin Lee for their technical help in this study. This work was supported by grant No. 2000-1-21300-001-2 from the Basic Research Program of the Korea Science and Engineering Foundation.",

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doi = "10.1016/S0006-8993(01)03056-6",

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AU - Kang, Tae Cheon

AU - Kim, Hyun Sook

AU - Seo, Myung Oak

AU - Choi, Soo Young

AU - Kwon, Oh Shin

AU - Baek, Nam In

AU - Lee, Hyeon Yong

AU - Won, Moo Ho

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PY - 2001/11/30

Y1 - 2001/11/30

N2 - In the present study, the distribution of glutamic acid decarboxylase (GAD) isoforms in the hippocampus of the Mongolian gerbil and its association with different sequelae of spontaneous seizure were investigated to identify the roles of balance of GAD isoforms in the epileptogenesis and the recovery mechanisms in these animals. The GAD67/GAD65 ratio in the hippocampus of pre-seizure seizure sensitive (SS) gerbil was ∼3.5-fold higher as compared to seizure resistant (SR) gerbil. Following seizure, this ratio shifted to the level of SR gerbils up to 12 h postical. Therefore, the mismatched GAD67/GAD65 ratio (imbalance of GAD isoform expressions) in the hippocampus of SS gerbil implies that GABAergic neurons may be highly activated in order to regulate the increased neuronal excitability. In addition, the alteration in this ratio after seizure may be the compensatory response for reduction of epileptic activity in this animal.

AB - In the present study, the distribution of glutamic acid decarboxylase (GAD) isoforms in the hippocampus of the Mongolian gerbil and its association with different sequelae of spontaneous seizure were investigated to identify the roles of balance of GAD isoforms in the epileptogenesis and the recovery mechanisms in these animals. The GAD67/GAD65 ratio in the hippocampus of pre-seizure seizure sensitive (SS) gerbil was ∼3.5-fold higher as compared to seizure resistant (SR) gerbil. Following seizure, this ratio shifted to the level of SR gerbils up to 12 h postical. Therefore, the mismatched GAD67/GAD65 ratio (imbalance of GAD isoform expressions) in the hippocampus of SS gerbil implies that GABAergic neurons may be highly activated in order to regulate the increased neuronal excitability. In addition, the alteration in this ratio after seizure may be the compensatory response for reduction of epileptic activity in this animal.

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KW - Epilepsy

KW - GAD65

KW - GAD67

KW - Gerbil

KW - Hippocampus

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The temporal alteration of GAD67/GAD65 ratio in the gerbil hippocampal complex following seizure

Abstract

Bibliographical note

Keywords

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