The temporal alteration of GAD67/GAD65 ratio in the gerbil hippocampal complex following seizure

Tae Cheon Kang, Hyun Sook Kim, Myung Oak Seo, Soo Young Choi, Oh Shin Kwon, Nam In Baek, Hyeon Yong Lee, Moo Ho Won

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41 Scopus citations


In the present study, the distribution of glutamic acid decarboxylase (GAD) isoforms in the hippocampus of the Mongolian gerbil and its association with different sequelae of spontaneous seizure were investigated to identify the roles of balance of GAD isoforms in the epileptogenesis and the recovery mechanisms in these animals. The GAD67/GAD65 ratio in the hippocampus of pre-seizure seizure sensitive (SS) gerbil was ∼3.5-fold higher as compared to seizure resistant (SR) gerbil. Following seizure, this ratio shifted to the level of SR gerbils up to 12 h postical. Therefore, the mismatched GAD67/GAD65 ratio (imbalance of GAD isoform expressions) in the hippocampus of SS gerbil implies that GABAergic neurons may be highly activated in order to regulate the increased neuronal excitability. In addition, the alteration in this ratio after seizure may be the compensatory response for reduction of epileptic activity in this animal.

Original languageEnglish
Pages (from-to)159-169
Number of pages11
JournalBrain Research
Issue number1-2
StatePublished - 30 Nov 2001

Bibliographical note

Funding Information:
The GAD65 (GAD-6) antibody developed by Dr D.I. Gottlieb was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa, Department of Biological Sciences, Iowa City, IA 52242, USA. The authors would like to thank Suek Han and Kyung Jin Lee for their technical help in this study. This work was supported by grant No. 2000-1-21300-001-2 from the Basic Research Program of the Korea Science and Engineering Foundation.


  • Dentate gyrus
  • Epilepsy
  • GAD65
  • GAD67
  • Gerbil
  • Hippocampus
  • Subiculum


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