The synthesis of tamoxifen-loaded albumin nanoparticles by homogenizers: Optimization and in vitro characterization

Maryam Sadat Safavi, Seyed Abbas Shojaosadati, Farid Abedin Dorkoosh, Hyun Ji Jo, Youngjoo Kwon, Kang Choon Lee, Hye Gyeong Yang, Eun Ji Park, Dong Hee Na

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The aim of this study was to develop mechanical homogenization processes to fabricate protein-based nanoparticles. The high-pressure homogenizer (HPH) and high-speed homogenizer (HSH); were used to encapsulate the hydrophobic drug, tamoxifen, in albumin nanoparticles. The results revealed that the rotational speed with HSH and the pressure with HPH were the main factors affecting the size, while increasing the residence time led to more homogenous nanoparticles. Seven homogenization cycles at 14917 psi and 8.24 min of mixing at 17360 rpm ensured a drug loading of 14.2 ± 1.9% and 11.6 ± 2.3% for HPH and HSH, respectively. We found a direct correlation between the obtained size and energy input and retention time with both homogenizing devices. The characteristics of the optimized nanoparticles were within the desired range to meet the requirements of intravenous injection. The surface morphology of the nanoparticles determined by transmission electron microscopy showed semi-spherical nanoparticle shapes. Further, the secondary structure of albumin in nanoparticles was determined via circular dichroism, which showed only slight structural changes versus native albumin, making it a promising, self-targeted drug delivery system. Finally, BT474 viability assays and western blot analysis showed the effectiveness of the tamoxifen-loaded albumin nanoparticles prepared via homogenization.

Original languageEnglish
Pages (from-to)20-30
Number of pages11
JournalJournal of Drug Delivery Science and Technology
Volume41
DOIs
StatePublished - Oct 2017

Bibliographical note

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

  • Albumin nanoparticles
  • High-pressure homogenizer
  • High-speed homogenizer
  • Response surface methodology
  • Tamoxifen

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