@article{c2853bf072b347d898c0849404022e3b,
title = "The role of NdgR in glycerol metabolism in Streptomyces coelicolor",
abstract = "Streptomyces, which produces many pharmaceutical antibiotics and anticancer agents, is a genus of soil-dwelling bacteria with numerous regulators that control both primary and secondary metabolism. NdgR is highly conserved in Streptomyces spp. and is known to be involved in antibiotic production, tolerance against shock and physical stress, nitrogen metabolism, leucine metabolism, and N-acetylglucosamine metabolism. As another function of NdgR, we report the involvement of NdgR in glycerol metabolism in S. coelicolor. Initially, a glycerol utilization operon containing gylCABX was found to be up-regulated in an ndgR deletion mutant (BG11) grown in N-acetylglucosamine solid minimal media compared with wild-type strain (M145). BG11 produced more antibiotics with a small amount of glycerol and increased glycerol utilization, yielding higher concentrations of lactate and acetate per cell. Moreover, fatty acid production was also changed in BG11 to produce longer chain fatty acids, phenolic compounds, alkanes, and fatty alcohols. Using a gel retardation assay, NdgR was found to bind the upstream region of gylC, working as a repressor. NdgR is a second regulator of a glycerol utilization operon, for which only one regulator, GylR was previously known.",
keywords = "Glycerol, N-Acetylglucosamine, NdgR, Regulator, Streptomyces coelicolor",
author = "Lee, {Bo Rahm} and Bhatia, {Shashi Kant} and Song, {Hun Suk} and Junyoung Kim and Wooseong Kim and Park, {Hyung yeon} and Yoon, {Jeong Jun} and Park, {Sung Hee} and Daehee Hwang and Kim, {Byung Gee} and Yang, {Yung Hun}",
note = "Funding Information: Funding The study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03033594 and NRF-2015M1A5A1037196), the Korea Institute of Energy Technology Evaluation and Planning (KETEP), and the Ministry of Trade, Industry and Energy (MOTIE) of the Republic of Korea (No. 20163010092150). This study was also partially supported by the Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (1201349190011). Funding Information: Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, Korea) was kindly appreciated. The study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03033594 and NRF-2015M1A5A1037196), the Korea Institute of Energy Technology Evaluation and Planning (KETEP), and the Ministry of Trade, Industry and Energy (MOTIE) of the Republic of Korea (No. 20163010092150). This study was also partially supported by the Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (1201349190011). The authors declare that they have no conflict of interest. Publisher Copyright: {\textcopyright} 2017, Springer-Verlag GmbH Germany.",
year = "2017",
month = oct,
doi = "10.1007/s00449-017-1813-z",
language = "English",
volume = "40",
pages = "1573--1580",
journal = "Bioprocess and Biosystems Engineering",
issn = "1615-7591",
publisher = "Springer Verlag",
number = "10",
}