The role of lipid peroxidation and glutathione on the glycochenodeoxycholic acid-induced cell death in primary cultured rat hepatocytes

S. Chu, W. Park, K. Lee, Y. Pae

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Intracellular accumulation of bile acids in the hepatocytes during cholestasis is thought to be pathogenic in cholestatic liver diseases. The objective of this study was to determine the role of lipid peroxidation and glutathione on the bile acid-induced hepatic cell death mechanism in primary cultured rat hepatocytes. To induce hepatic cell death, we incubated primary cultured rat hepatocytes with glycochenodeoxycholic acid (GCDC; 0~400 μM) for 3 hours. In electron microscopic examination and agarose gel electrophoresis, low concentration of GCDC treatment mainly induced apoptotic feature. Whereas 400 μM GCDC treated cells demonstrated both apoptosis and necrosis. Lipid peroxidation was increased dose-dependently in GCDC treated hepatocyte. And this was also accompanied by decreased glutathione. Therefore, oxygen free radical damage may play a partial role in GCDC-induced hepatic cell death.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalKorean Journal of Physiology and Pharmacology
Volume4
Issue number2
StatePublished - 2000

Keywords

  • Apoptosis
  • Glutathione
  • Glycochenodeoxycholic acid
  • Hepatocyte
  • Lipid peroxidation

Fingerprint

Dive into the research topics of 'The role of lipid peroxidation and glutathione on the glycochenodeoxycholic acid-induced cell death in primary cultured rat hepatocytes'. Together they form a unique fingerprint.

Cite this