The RING-H2-finger protein APC11 as a target of hydrogen peroxide

Tong Shin Chang, Woojin Jeong, Duck Yeon Lee, Chun Seok Cho, Sue Goo Rhee

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The anaphase-promoting complex (APC) is a ubiquitin-protein ligase (E3) that targets cell cycle regulators such as cyclin B and securin for degradation. The APC11 subunit functions as the catalytic core of this complex and mediates the transfer of ubiquitin from a ubiquitin-conjugating enzyme (E2) to the substrate. APC11 contains a RING-H2-finger domain, which includes one histidine and seven cysteine residues that coordinate two Zn2+ ions. We now show that exposure of purified APC11 to H2O2 (0.1 to 1 mM) induced the release of bound zinc as a result of the oxidation of cysteine residues. It also impaired the physical interaction between APC11 and the E2 enzyme Ubc4 as well as inhibited the ubiquitination of cyclin B1 by APC11. The release of HeLa cells from metaphase arrest in the presence of exogenous H 2O2 inhibited the ubiquitination of cyclin B1 as well as the degradation of cyclin B1 and securin that were apparent in the absence of H2O2. The presence of H2O2 also blocked the co-immunoprecipitation of Ubc4 with APC11 and delayed the exit of cells from mitosis. Inhibition of APC11 function by H2O2 thus likely contributes to the delay in cell cycle progression through mitosis that is characteristic of cells subjected to oxidative stress.

Original languageEnglish
Pages (from-to)521-530
Number of pages10
JournalFree Radical Biology and Medicine
Volume37
Issue number4
DOIs
StatePublished - 15 Aug 2004

Keywords

  • 4-acetamido- 4′-maleimidylstilbene-2,2′-disulfonate
  • AMS
  • anaphase-promoting complex
  • Anaphase-promoting complex 11
  • APC
  • Cysteine oxidation
  • Free radicals
  • HA
  • Hydrogen peroxide
  • RING finger
  • Ubiquitin-protein ligase
  • Zinc release

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