The matrix metalloproteinase-7 regulates the extracellular shedding of syndecan-2 from colon cancer cells

Sojoong Choi, Jin Yung Kim, Jun Hyoung Park, Seung Teak Lee, Inn Oc Han, Eok Soo Oh

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The cell surface heparan sulfate proteoglycan syndecan-2 regulates the activation of matrix metalloproteinase-7 (MMP-7) as a docking receptor. Here, we demonstrate the role of MMP-7 on syndecan-2 shedding in colon cancer cells. Western blot analysis showed that shed syndecan-2 was found in the culture media from various colon cancer cells. Overexpression of MMP-7 enhanced syndecan-2 shedding, whereas the opposite was true when MMP-7 levels were knocked-down using small inhibitory RNAs. Consistently, HT29 cells treated with MMP-7, but neither MMP-2 nor MMP-9, showed increased shed syndecan-2 in a time- and concentration-dependent manner. Furthermore, MALDI-TOF MS analysis and N-terminal amino acid sequencing revealed that MMP-7 cleaved both recombinant syndecan-2 and an endogenously glycosylated syndecan-2 ectodomain in the N-terminus at Leu 149 residue in vitro. Taken together, the data suggest that MMP-7 directly mediates shedding of syndecan-2 from colon cancer cells.

Original languageEnglish
Pages (from-to)1260-1264
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume417
Issue number4
DOIs
StatePublished - 27 Jan 2012

Keywords

  • Colon cancer
  • Matrix metalloproteinase-7
  • Shedding
  • Syndecan-2

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