The isolation and in situ identification of MSCs residing in loose connective tissues using a niche-preserving organ culture system

Min Young Choi, Hyeong In Kim, Young Il Yang, Jong Tae Kim, Soo Hwa Jang, Chung Mu Park, Won Hee Jang, Young Chul Youn, Soon Ho Cheong, Chang Soo Choi, Dae Kyeong Kim, Seung Jin Lee

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) have been discovered in a multitude of organs, but their distribution and identity are still uncertain Furthermore, loose connective tissue (LCT) is dispersed throughout virtually all organs, but its biological role in tissue homeostasis is unclear Here, we describe a unique organ culture system to explore the omnipresence and in situ identity of MSCs among the LCTs This culture system included the use of the fibrin hydrogel coupled with dynamic culture conditions, using native LCTs obtained from various organs as starting materials This culture allowed MSC outgrowth into the hydrogel to be robustly supported, while maintaining the structural integrity of LCTs during in vitro culture Subcultured outgrown cells fulfilled the minimal requirements for defining MSCs on the basis of clonogenicity, multipotency, and immunophenotypic characteristics In vitro label-retaining assay demonstrated that the numbers of mobilized and proliferated cells in situ increased in the pericapillary region and expressed both MSCs and pericytes markers, indicating that the in situ identity of MSCs represents a certain population of pericapillary pericytes Our results indicate that this culture system affords a unique strategy for both isolating MSCs and recapitulating their niche in LCTs

Original languageEnglish
Pages (from-to)4469-4479
Number of pages11
JournalBiomaterials
Volume33
Issue number18
DOIs
StatePublished - Jun 2012

Keywords

  • Fibrin
  • Loose connective tissues
  • Mesenchymal stem cells
  • Organ culture
  • Pericytes
  • Stem cell niche

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