The Involvement of K+ Channels and the Possible Pathway of EDHF in the Rabbit Femoral Artery

Seong Chun Kwon, Wook Bum Pyun, Gi Young Park, Hee Kyung Choi, Kwang Se Paik, Bok Soon Kang

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5 Scopus citations


Experiments were designed to characterize the cellular mechanisms of action of endothelium-derived vasodilator substances in the rabbit femoral artery. Acetylcholine (ACh, 10-8-10-5 M) induced a concentration-dependent relaxation of isolated endothelium-intact arterial rings precontracted with norepinephrine (NE, 10-6 M). The ACh-induced response was abolished by the removal of endothelium. NG-nitro-L-arginine (L-NAME, 10-4 M), an inhibitor of NO synthase, partially inhibited ACh-induced endothelium-dependent relaxation, whereas indomethacin (10-5 M) showed no effect on ACh-induced relaxation. 25 mM KCl partially inhibited ACh-induced relaxation by shifting the concentration-response curve and abolished the response when combined with L-NAME and NE. In the presence of L-NAME, ACh-induced relaxation was unaffected by glibenclamide (10-5 M) but significantly reduced by apamin (10-5 M), and almost completely blocked by tetraethylammnonium (TEA, 10-3 M), iberiotoxin (10-7 M) and 4-aminopyridine (4-AP, 5 × 10-3 M). The cytochrome P450 inhibitors, 7-ethoxyresorufin (7-ER, 10-5 M) and miconazole (10-5 M) also significantly inhibited ACh-induced relaxation. Ouabain (10-6 M), an inhibitor of Na+ , K+ -ATPase, or K+ -free solution, also significantly inhibited ACh-induced relaxation. ACh-induced relaxation was not significantly inhibited by 18- α-glycyrrhetinic acid (18 α-GA, 10-4 M). These results of this study indicate that ACh-induced endothelium-dependent relaxation of the rabbit femoral artery occurs via a mechanism that involves activation of Na+, K+ -ATPase and/or activation of both the voltage-gated K+ channel (Kv) and the large-conductance, Ca2+ -activated K+ channel (BKCa). The results further suggest that EDHF released by ACh may be a cytochrome P450 product.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalYonsei Medical Journal
Issue number4
StatePublished - 1999


  • Acetylcholine
  • EDHF
  • Endothelium-clependent relaxation
  • K channel
  • Rabbit femoral artery


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