TY - JOUR
T1 - The immunohistochemical patterns of calcification-related molecules in the epidermis and dermis of the zebrafish (Danio rerio)
AU - Hong, Yeon Ju
AU - Choi, You Won
AU - Myung, Ki Bum
AU - Choi, Hae Young
PY - 2011/8
Y1 - 2011/8
N2 - Background: The scales of bony fish represent a significant reservoir of calcium and calcification of the elasmoid scale is known to be associated with deposition of mineral crystals from the epidermis to dermis. However, little is known about the exact mechanisms of calcium deposition, mobilization and regeneration occurring in the zebrafish skin. Objective: The purpose of this study was to investigate the expression of calcification-related molecular mediators in both the epidermis and dermis of the zebrafish (Danio rerio), using immunohistochemical study. Methods: We examined the skin of zebrafish in four populations of different ages (i.e. 20 days post-fertilization (dpf), 35 dpf, 50 dpf, and the adult zebrafish), using several immunohistochemical markers, including bone morphogenetic protein 4 (BMP-4), β-catenin, osteocalcin, osteopontin and osteonectin. Results: BMP-4, osteopontin and osteonectin were moderately expressed in the epidermis of zebrafish after 35 dpf. Also, some of the cells in the upper dermis showed strong positivity for BMP-4, osteocalcin, osteopontin and osteonetin. Conclusion: Our results suggest that BMP-4, osteocalcin, osteopontin and osteonectin may play a role in the process of calcification of the elasmoid scale.
AB - Background: The scales of bony fish represent a significant reservoir of calcium and calcification of the elasmoid scale is known to be associated with deposition of mineral crystals from the epidermis to dermis. However, little is known about the exact mechanisms of calcium deposition, mobilization and regeneration occurring in the zebrafish skin. Objective: The purpose of this study was to investigate the expression of calcification-related molecular mediators in both the epidermis and dermis of the zebrafish (Danio rerio), using immunohistochemical study. Methods: We examined the skin of zebrafish in four populations of different ages (i.e. 20 days post-fertilization (dpf), 35 dpf, 50 dpf, and the adult zebrafish), using several immunohistochemical markers, including bone morphogenetic protein 4 (BMP-4), β-catenin, osteocalcin, osteopontin and osteonectin. Results: BMP-4, osteopontin and osteonectin were moderately expressed in the epidermis of zebrafish after 35 dpf. Also, some of the cells in the upper dermis showed strong positivity for BMP-4, osteocalcin, osteopontin and osteonetin. Conclusion: Our results suggest that BMP-4, osteocalcin, osteopontin and osteonectin may play a role in the process of calcification of the elasmoid scale.
KW - Calcification
KW - Elasmoid scale
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=80052091055&partnerID=8YFLogxK
U2 - 10.5021/ad.2011.23.3.299
DO - 10.5021/ad.2011.23.3.299
M3 - Article
C2 - 21909199
AN - SCOPUS:80052091055
SN - 1013-9087
VL - 23
SP - 299
EP - 303
JO - Annals of Dermatology
JF - Annals of Dermatology
IS - 3
ER -