The extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin

Mi Jung Kwon; Yeonhee Kim; Youngsil Choi; So Hyun Kim; Sungsu Park; Innoc Han; Duk Hee Kang; Eok Soo Oh

doi:10.1016/j.bbrc.2012.12.155

The extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin

Mi Jung Kwon
, Yeonhee Kim
, Youngsil Choi
, So Hyun Kim
, Sungsu Park
, Innoc Han
, Duk Hee Kang
, Eok Soo Oh

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The cell surface heparan sulfate proteoglycan, syndecan-2, is known to play an important role in the tumorigenic activity of colon cancer cells, but the function of its extracellular domain is not yet clear. Cell spreading assays showed that HCT116 human colon cancer cells attached and spread better on fibronectin compared to the other tested extracellular matrixes (ECMs). Notably, syndecan-2 overexpression enhanced the spreading of HCT116 cells on fibronectin, and the opposite effects were observed when syndecan-2 expression was reduced. In addition, an oligomerization-defective syndecan-2 mutant failed to increase cell-ECM interactions and adhesion-related syndecan-2 functions, including migration. Furthermore, analyses using a microfabricated post array detector system revealed that syndecan-2, but not the oligomerization-defective mutant, enhanced the interaction affinity of HCT116 cells on fibronectin. Taken together, these results suggest that the extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin.

Original language	English
Pages (from-to)	415-420
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	431
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2012.12.155
State	Published - 15 Feb 2013

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2010-0026103) and a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (A120071).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell migration
Colon cancer
Extracellular matrix
Syndecan-2

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10.1016/j.bbrc.2012.12.155

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title = "The extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin",

abstract = "The cell surface heparan sulfate proteoglycan, syndecan-2, is known to play an important role in the tumorigenic activity of colon cancer cells, but the function of its extracellular domain is not yet clear. Cell spreading assays showed that HCT116 human colon cancer cells attached and spread better on fibronectin compared to the other tested extracellular matrixes (ECMs). Notably, syndecan-2 overexpression enhanced the spreading of HCT116 cells on fibronectin, and the opposite effects were observed when syndecan-2 expression was reduced. In addition, an oligomerization-defective syndecan-2 mutant failed to increase cell-ECM interactions and adhesion-related syndecan-2 functions, including migration. Furthermore, analyses using a microfabricated post array detector system revealed that syndecan-2, but not the oligomerization-defective mutant, enhanced the interaction affinity of HCT116 cells on fibronectin. Taken together, these results suggest that the extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin.",

keywords = "Cell migration, Colon cancer, Extracellular matrix, Syndecan-2",

author = "Kwon, \{Mi Jung\} and Yeonhee Kim and Youngsil Choi and Kim, \{So Hyun\} and Sungsu Park and Innoc Han and Kang, \{Duk Hee\} and Oh, \{Eok Soo\}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2010-0026103) and a grant of the Korean Health Technology R\&D Project, Ministry of Health \& Welfare, Republic of Korea. (A120071).",

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T1 - The extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin

AU - Kwon, Mi Jung

AU - Kim, Yeonhee

AU - Choi, Youngsil

AU - Kim, So Hyun

AU - Park, Sungsu

AU - Han, Innoc

AU - Kang, Duk Hee

AU - Oh, Eok Soo

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MEST) (No. 2010-0026103) and a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (A120071).

PY - 2013/2/15

Y1 - 2013/2/15

N2 - The cell surface heparan sulfate proteoglycan, syndecan-2, is known to play an important role in the tumorigenic activity of colon cancer cells, but the function of its extracellular domain is not yet clear. Cell spreading assays showed that HCT116 human colon cancer cells attached and spread better on fibronectin compared to the other tested extracellular matrixes (ECMs). Notably, syndecan-2 overexpression enhanced the spreading of HCT116 cells on fibronectin, and the opposite effects were observed when syndecan-2 expression was reduced. In addition, an oligomerization-defective syndecan-2 mutant failed to increase cell-ECM interactions and adhesion-related syndecan-2 functions, including migration. Furthermore, analyses using a microfabricated post array detector system revealed that syndecan-2, but not the oligomerization-defective mutant, enhanced the interaction affinity of HCT116 cells on fibronectin. Taken together, these results suggest that the extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin.

AB - The cell surface heparan sulfate proteoglycan, syndecan-2, is known to play an important role in the tumorigenic activity of colon cancer cells, but the function of its extracellular domain is not yet clear. Cell spreading assays showed that HCT116 human colon cancer cells attached and spread better on fibronectin compared to the other tested extracellular matrixes (ECMs). Notably, syndecan-2 overexpression enhanced the spreading of HCT116 cells on fibronectin, and the opposite effects were observed when syndecan-2 expression was reduced. In addition, an oligomerization-defective syndecan-2 mutant failed to increase cell-ECM interactions and adhesion-related syndecan-2 functions, including migration. Furthermore, analyses using a microfabricated post array detector system revealed that syndecan-2, but not the oligomerization-defective mutant, enhanced the interaction affinity of HCT116 cells on fibronectin. Taken together, these results suggest that the extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin.

KW - Cell migration

KW - Colon cancer

KW - Extracellular matrix

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The extracellular domain of syndecan-2 regulates the interaction of HCT116 human colon carcinoma cells with fibronectin

Abstract

Bibliographical note

UN SDGs

Keywords

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