The expressions of TGF-β1and IL-10 in cultured fibroblasts after ALA-IPL photodynamic treatment

Ji Yeon Byun, Ga Youn Lee, Hae Young Choi, Ki Bum Myung, You Won Choi

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29 Scopus citations

Abstract

Background: Topical photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA) was originally used for treating superficial skin tumors. The application of PDT to other inflammatory dermatoses like acne vulgaris, psoriasis, granuloma annulare, localized scleroderma and lichen sclerosus has recently been introduced. However, the underlying mechanisms are not well understood. We've previously reported the induction of tumor growth factor (TGF)- β1 and interleukin (IL)-10 after PDT with ALA and intense pulsed light (IPL) in cultured HaCaT cells. Objective: The purpose of this study was to investigate the expressions of TGF-β1 and IL-10 in cultured fibroblasts after PDT with using ALA and IPL. Methods: Cultured fibroblasts were treated with ALA-IPL PDT (1 μ mol/L of ALA; 0,4, 8 and 12 J/cm2 of IPL). The expressions of TGF-β1 and IL-10 were investigated by reverse transcription-polymerase chain reaction and enzyme linked immunosorbent assay. Results: TGF-β1 mRNAand protein were reduced down to 0.52-and 0.63-fold, respectively, after PDT and the IL-10 protein was increased up to 2.74-fold after PDT. Conclusion: The reduction of TGF-β1 was prominent after PDT and so an antisclerotic effect can be expected after PDT. The induction of IL-10 may contribute to the anti-inflammatory effect, which explains the therapeutic benefit of PDT for inflammatory dermatoses.

Original languageEnglish
Pages (from-to)19-22
Number of pages4
JournalAnnals of Dermatology
Volume23
Issue number1
DOIs
StatePublished - Feb 2011

Keywords

  • Aminolevulinic acid
  • Fibroblasts
  • IL-10
  • Photodynamic therapy
  • Tgf-β

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