The effect of umbilical cord blood derived mesenchymal stem cells in monocrotaline-induced pulmonary artery hypertension rats

Hyeryon Lee, Jae Chul Lee, Jung Hyun Kwon, Kwan Chang Kim, Min Sun Cho, Yoon Sun Yang, Wonil Oh, Soo Jin Choi, Eun Seok Seo, Sang Joon Lee, Tae Jun Wang, Young Mi Hong

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Pulmonary arterial hypertension (PAH) causes right ventricular failure due to a gradual increase in pulmonary vascular resistance. The purposes of this study were to confirm the engraftment of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) placed in the correct place in the lung and research on changes of hemodynamics, pulmonary pathology, immunomodulation and several gene expressions in monocrotaline (MCT)- induced PAH rat models after hUCB-MSCs transfusion. The rats were grouped as follows: the control (C) group; the M group (MCT 60 mg/kg); the U group (hUCB-MSCs transfusion). They received transfusions via the external jugular vein a week after MCT injection. The mean right ventricular pressure (RVP) was significantly reduced in the U group after the 2 week. The indicators of RV hypertrophy were significantly reduced in the U group at week 4. Reduced medial wall thickness in the pulmonary arteriole was noted in the U group at week 4. Reduced number of intra-acinar muscular pulmonary arteries was observed in the U group after 2 week. Protein expressions such as endothelin (ET)-1, endothelin receptor A (ERA), endothelial nitric oxide synthase (eNOS) and matrix metalloproteinase (MMP)-2 significantly decreased at week 4. The decreased levels of ERA, eNOS and MMP-2 immunoreactivity were noted by immnohistochemical staining. After hUCB-MSCs were administered, there were the improvement of RVH and mean RVP. Reductions in several protein expressions and immunomodulation were also detected. It is suggested that hUCB-MSCs may be a promising therapeutic option for PAH.

Original languageEnglish
Pages (from-to)576-585
Number of pages10
JournalJournal of Korean Medical Science
Volume30
Issue number5
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 The Korean Academy of Medical Sciences.

Keywords

  • Cord blood stem cell transfusion
  • Gene Expression
  • Hypertension
  • Monocrotaline
  • Pulmonary

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