The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models

Ji Seon Chae; Hyunju Park; So Hee Ahn; Eun Chong Han; Yoonjin Lee; Youn Jin Kim; Eun Jin Ahn; Hye Won Oh; Hyun Jung Lee; Chulhee Choi; Youn Hee Choi; Won Joong Kim

doi:10.3390/pharmaceutics15020553

The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models

Ji Seon Chae
, Hyunju Park
, So Hee Ahn
, Eun Chong Han
, Yoonjin Lee
, Youn Jin Kim
, Eun Jin Ahn
, Hye Won Oh
, Hyun Jung Lee
, Chulhee Choi
, Youn Hee Choi
, Won Joong Kim

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.

Original language	English
Article number	553
Journal	Pharmaceutics
Volume	15
Issue number	2
DOIs	https://doi.org/10.3390/pharmaceutics15020553
State	Published - Feb 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Keywords

NFκB
allodynia
chronic post-ischemic pain model
complex regional pain syndrome (CRPS)
exosome loaded with super-repressor IκB
exosomes
inflammation
neuropathic pain

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title = "The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models",

abstract = "Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.",

keywords = "NFκB, allodynia, chronic post-ischemic pain model, complex regional pain syndrome (CRPS), exosome loaded with super-repressor IκB, exosomes, inflammation, neuropathic pain",

author = "Chae, \{Ji Seon\} and Hyunju Park and Ahn, \{So Hee\} and Han, \{Eun Chong\} and Yoonjin Lee and Kim, \{Youn Jin\} and Ahn, \{Eun Jin\} and Oh, \{Hye Won\} and Lee, \{Hyun Jung\} and Chulhee Choi and Choi, \{Youn Hee\} and Kim, \{Won Joong\}",

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AU - Chae, Ji Seon

AU - Park, Hyunju

AU - Ahn, So Hee

AU - Han, Eun Chong

AU - Lee, Yoonjin

AU - Kim, Youn Jin

AU - Ahn, Eun Jin

AU - Oh, Hye Won

AU - Lee, Hyun Jung

AU - Choi, Chulhee

AU - Choi, Youn Hee

AU - Kim, Won Joong

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AB - Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.

KW - NFκB

KW - allodynia

KW - chronic post-ischemic pain model

KW - complex regional pain syndrome (CRPS)

KW - exosome loaded with super-repressor IκB

KW - exosomes

KW - inflammation

KW - neuropathic pain

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DO - 10.3390/pharmaceutics15020553

M3 - Article

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SN - 1999-4923

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ER -

The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models

Abstract

Bibliographical note

Keywords

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