The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models

Ji Seon Chae, Hyunju Park, So Hee Ahn, Eun Chong Han, Yoonjin Lee, Youn Jin Kim, Eun Jin Ahn, Hye Won Oh, Hyun Jung Lee, Chulhee Choi, Youn Hee Choi, Won Joong Kim

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.

Original languageEnglish
Article number553
JournalPharmaceutics
Volume15
Issue number2
DOIs
StatePublished - Feb 2023

Bibliographical note

Funding Information:
Chulhee Choi is the founder and a shareholder of ILIAS Biologics Inc. This study is partly supported by a grant from ILIAS Biologics Inc.

Funding Information:
This research was funded by the National Research Foundation of Korea (NRF), grant number NRF-2020R1A5A2019210 (YHC).

Publisher Copyright:
© 2023 by the authors.

Keywords

  • NFκB
  • allodynia
  • chronic post-ischemic pain model
  • complex regional pain syndrome (CRPS)
  • exosome loaded with super-repressor IκB
  • exosomes
  • inflammation
  • neuropathic pain

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