The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models

Ji Seon Chae; Hyunju Park; So Hee Ahn; Eun Chong Han; Yoonjin Lee; Youn Jin Kim; Eun Jin Ahn; Hye Won Oh; Hyun Jung Lee; Chulhee Choi; Youn Hee Choi; Won Joong Kim

doi:10.3390/pharmaceutics15020553

The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models

Ji Seon Chae, Hyunju Park, So Hee Ahn, Eun Chong Han, Yoonjin Lee, Youn Jin Kim, Eun Jin Ahn, Hye Won Oh, Hyun Jung Lee, Chulhee Choi, Youn Hee Choi, Won Joong Kim

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.

Original language	English
Article number	553
Journal	Pharmaceutics
Volume	15
Issue number	2
DOIs	https://doi.org/10.3390/pharmaceutics15020553
State	Published - Feb 2023

Bibliographical note

Funding Information:
Chulhee Choi is the founder and a shareholder of ILIAS Biologics Inc. This study is partly supported by a grant from ILIAS Biologics Inc.

Funding Information:
This research was funded by the National Research Foundation of Korea (NRF), grant number NRF-2020R1A5A2019210 (YHC).

Publisher Copyright:
© 2023 by the authors.

Keywords

NFκB
allodynia
chronic post-ischemic pain model
complex regional pain syndrome (CRPS)
exosome loaded with super-repressor IκB
exosomes
inflammation
neuropathic pain

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title = "The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models",

abstract = "Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.",

keywords = "NFκB, allodynia, chronic post-ischemic pain model, complex regional pain syndrome (CRPS), exosome loaded with super-repressor IκB, exosomes, inflammation, neuropathic pain",

author = "Chae, {Ji Seon} and Hyunju Park and Ahn, {So Hee} and Han, {Eun Chong} and Yoonjin Lee and Kim, {Youn Jin} and Ahn, {Eun Jin} and Oh, {Hye Won} and Lee, {Hyun Jung} and Chulhee Choi and Choi, {Youn Hee} and Kim, {Won Joong}",

note = "Funding Information: Chulhee Choi is the founder and a shareholder of ILIAS Biologics Inc. This study is partly supported by a grant from ILIAS Biologics Inc. Funding Information: This research was funded by the National Research Foundation of Korea (NRF), grant number NRF-2020R1A5A2019210 (YHC). Publisher Copyright: {\textcopyright} 2023 by the authors.",

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month = feb,

doi = "10.3390/pharmaceutics15020553",

language = "English",

volume = "15",

journal = "Pharmaceutics",

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AU - Chae, Ji Seon

AU - Park, Hyunju

AU - Ahn, So Hee

AU - Han, Eun Chong

AU - Lee, Yoonjin

AU - Kim, Youn Jin

AU - Ahn, Eun Jin

AU - Oh, Hye Won

AU - Lee, Hyun Jung

AU - Choi, Chulhee

AU - Choi, Youn Hee

AU - Kim, Won Joong

N1 - Funding Information: Chulhee Choi is the founder and a shareholder of ILIAS Biologics Inc. This study is partly supported by a grant from ILIAS Biologics Inc. Funding Information: This research was funded by the National Research Foundation of Korea (NRF), grant number NRF-2020R1A5A2019210 (YHC). Publisher Copyright: © 2023 by the authors.

PY - 2023/2

Y1 - 2023/2

N2 - Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.

AB - Complex regional pain syndrome (CRPS) is a condition associated with neuropathic pain that causes significant impairment of daily activities and functioning. Nuclear factor kappa B (NFκB) is thought to play an important role in the mechanism of CRPS. Recently, exosomes loaded with super-repressor inhibitory kappa B (Exo-srIκB, IκB; inhibitor of NFκB) have been shown to have potential anti-inflammatory effects in various inflammatory disease models. We investigated the therapeutic effect of Exo-srIκB on a rodent model with chronic post-ischemia pain (CPIP), a representative animal model of Type I CRPS. After intraperitoneal injection of a vehicle, Exo-srIκB, and pregabalin, the paw withdrawal threshold (PWT) was evaluated up to 48 h. Administration of Exo-srIκB increased PWT compared to the vehicle and pregabalin, and the relative densities of p-IκB and IκB showed significant changes compared to the vehicle 24 h after Exo-srIκB injection. The levels of several cytokines and chemokines were reduced by the administration of Exo-srIκB in mice with CPIP. In conclusion, our results showed more specifically the role of NFκB in the pathogenesis of CRPS and provided a theoretical background for novel treatment options for CRPS.

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KW - allodynia

KW - chronic post-ischemic pain model

KW - complex regional pain syndrome (CRPS)

KW - exosome loaded with super-repressor IκB

KW - exosomes

KW - inflammation

KW - neuropathic pain

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DO - 10.3390/pharmaceutics15020553

M3 - Article

AN - SCOPUS:85149147645

SN - 1999-4923

VL - 15

JO - Pharmaceutics

JF - Pharmaceutics

IS - 2

M1 - 553

ER -

The Effect of Super-Repressor IkB-Loaded Exosomes (Exo-srIκBs) in Chronic Post-Ischemia Pain (CPIP) Models

Abstract

Bibliographical note

Keywords

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