The effect of cytokine mediators on prostaglandin inhibition by human decidual cells

OBJECTIVE: The purpose of this study was to determine the inhibitory effects of anti-interleukin-1β and transforming growth factor-β₂ on the production of interleukin-1β and prostaglandin E₂ by human decidual cells. STUDY DESIGN: Decidual membranes were obtained from 30 patients between 37 and 40 weeks' gestation. Decidual cells were isolated and grown in culture. Incubations were conducted with lipopolysaccharide as a control lipopolysaccharide plus anti-interleukin-1β, and lipopolysaccharide plus transforming growth factorβ₂, respectively. Interleukin-1β and prostaglandin E₂ were measured by enzyme-linked immunosorbent assay and radioimmunoassay. RESULTS: At the end of a 24-hour culture with lipopolysaccharide plus anti-interleukin-1β, anti-interleukin-1β decreased the lipopolysaccharide-induced prostaglandin E₂ production significantly at the concentrations of 50 ng/mL, 100 ng/mL, and 500 ng/mL (P < .05) and also decreased the lipopolysaccharide-induced interleukin-1β production significantly at the concentrations of 10 ng/mL, 50 ng/mL, and 100 ng/mL (P < .05). In the medium containing lipopolysaccharide plus transforming growth factor-β₂, transforming growth factor-β₂ also decreased the lipopolysaccharide-induced prostaglandin E₂ production significantly at the concentrations of 1 ng/mL and 10 ng/mL (P < .05) but increased the lipopolysaccharide-induced interleukin-1β production significantly at the concentration of 1 ng/mL (P < .05). CONCLUSIONS: Lipopolysaccharide-induced prostaglandin E₂ production by human decidual cells in vitro may be prevented by anti-interleukin-1β and transforming growth factor-β₂, and interleukin-1β production may be prevented by anti-interleukin-1β.