The effect of antioxidants on glycated albumin-induced cytotoxicity in bovine retinal pericytes

Jaetaek Kim, Kwang seok Kim, Jong wook Shinn, Yeon sahng Oh, Soon hyun Shinn, Heung tae Kim, Inho Jo

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32 Scopus citations


Loss of retinal pericytes is the initial deficit in the early stage of diabetic retinopathy. Glycated albumin (GA) forms under hyperglycemic conditions and exists in the retinal blood vessels of diabetic patients with retinopathy. In this study, using a 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) reduction test, we investigated whether GA induces cytotoxicity in cultured bovine retinal pericytes, and whether the antioxidants, L-ascorbic acid, Trolox, and probucol, provide any protection from GA-mediated cytotoxicity. GA induced pericyte death in a dose-dependent manner. With increasing time, GA-induced cytotoxicity also increased despite no strict time dependence. Furthermore, this cell death was found to be mediated both by apoptosis, which was confirmed by apoptosis-specific fluorescent staining of nuclei and cell membranes, and mitochondrial damage, as elucidated by electron microscopy. All three antioxidants used in this study partially protected against GA-induced pericyte death, suggesting that oxidative stress plays a role in GA-induced pericyte death. The results indicate that GA induces cell death in cultured bovine retinal pericytes, and that certain antioxidants may reduce this cytotoxicity.

Original languageEnglish
Pages (from-to)1010-1016
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number4
StatePublished - 2002


  • Antioxidants
  • Apoptosis
  • Cytotoxicity
  • Glycated albumin
  • Retinal pericytes


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