We present a microfabricated three-dimensional (3-D) hybrid biopolymer micro cantilever which can measure the contractile force of cardiomyocytes and analyze the force dependence on different types of surface. To make different conditions of the cell seeding surface, we fabricated the specific type of cantilever which was grooved on the surface. The presented cantilever was facilitated to measure bending of the cantilever and to calculate the contractile force of cardiomyocytes. Also, we demonstrate the dependence of the morphology for the cardiomyocytes seeded on a plain surface and a grooved surface. Finally, we show the dependence of force for the cardiomyocytes on a different surface.