The complete mitochondrial genome of the rayfish Raja porosa (Chondrichthyes, Rajidae)

Il Chan Kim; Sang Oun Jung; Young Mi Lee; Changjoo Lee; Joong Ki Park; Jae Seong Lee

doi:10.1080/10425170500087975

The complete mitochondrial genome of the rayfish Raja porosa (Chondrichthyes, Rajidae)

Il Chan Kim, Sang Oun Jung, Young Mi Lee, Changjoo Lee, Joong Ki Park, Jae Seong Lee

Divison of EcoScience

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We isolated mitochondrial DNA from the rayfish Raja porosa by long-polymerase chain reaction (Long-PCR) with conserved primers, and sequenced it by primer walking method using flanking sequences as sequencing primers. R. porosa mitochondrial DNA consists of 16,972 bp and its structural organization is conserved in comparison with other fishes and mammals. Based on the mitochondrial cytochrome b (cyt b) sequence, the phylogenetic position of R. porosa among cartilaginous fishes was inferred using different phylogenetic methods (ML-based quartet puzzling, Neighbor-joining (NJ) and Bayesian approaches). In this paper, we report the characteristics of the R. porosa mitochondrial genome including structural organization, base composition of rRNAs, tRNAs and protein-encoding genes and characteristics of mitochondrial tRNAs. These findings are applicable to comparative mitogenomics of R. porosa with other related taxa.

Original language	English
Pages (from-to)	187-194
Number of pages	8
Journal	DNA Sequence - Journal of DNA Sequencing and Mapping
Volume	16
Issue number	3
DOIs	https://doi.org/10.1080/10425170500087975
State	Published - Jun 2005

Keywords

Cartilaginous fish
Long-polymerase chain reaction
Mitochondrial genome
Raja porosa
Rayfish

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10.1080/10425170500087975

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abstract = "We isolated mitochondrial DNA from the rayfish Raja porosa by long-polymerase chain reaction (Long-PCR) with conserved primers, and sequenced it by primer walking method using flanking sequences as sequencing primers. R. porosa mitochondrial DNA consists of 16,972 bp and its structural organization is conserved in comparison with other fishes and mammals. Based on the mitochondrial cytochrome b (cyt b) sequence, the phylogenetic position of R. porosa among cartilaginous fishes was inferred using different phylogenetic methods (ML-based quartet puzzling, Neighbor-joining (NJ) and Bayesian approaches). In this paper, we report the characteristics of the R. porosa mitochondrial genome including structural organization, base composition of rRNAs, tRNAs and protein-encoding genes and characteristics of mitochondrial tRNAs. These findings are applicable to comparative mitogenomics of R. porosa with other related taxa.",

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author = "Kim, {Il Chan} and Jung, {Sang Oun} and Lee, {Young Mi} and Changjoo Lee and Park, {Joong Ki} and Lee, {Jae Seong}",

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AU - Lee, Changjoo

AU - Park, Joong Ki

AU - Lee, Jae Seong

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AB - We isolated mitochondrial DNA from the rayfish Raja porosa by long-polymerase chain reaction (Long-PCR) with conserved primers, and sequenced it by primer walking method using flanking sequences as sequencing primers. R. porosa mitochondrial DNA consists of 16,972 bp and its structural organization is conserved in comparison with other fishes and mammals. Based on the mitochondrial cytochrome b (cyt b) sequence, the phylogenetic position of R. porosa among cartilaginous fishes was inferred using different phylogenetic methods (ML-based quartet puzzling, Neighbor-joining (NJ) and Bayesian approaches). In this paper, we report the characteristics of the R. porosa mitochondrial genome including structural organization, base composition of rRNAs, tRNAs and protein-encoding genes and characteristics of mitochondrial tRNAs. These findings are applicable to comparative mitogenomics of R. porosa with other related taxa.

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KW - Mitochondrial genome

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The complete mitochondrial genome of the rayfish Raja porosa (Chondrichthyes, Rajidae)

Abstract

Keywords

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