The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolism

Sook Lee Hyun, Gyun Kang Sung, Seob Bae Seung, Kyu Lim Jae, Yona Cho, Jae Kim Yun, Ho Jeon Jeong, Sun Shin Cha, Kyoung Kwon Kae, Hyung Tae Kim, Cheol Joo Park, Hee Wook Lee, Il Kim Seung, Jongsik Chun, Rita R. Colwell, Sang Jin Kim, Jung Hyun Lee

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

Members of the genus Thermococcus, sulfur-reducing hyperthermophilic archaea, are ubiquitously present in various deep-sea hydrothermal vent systems and are considered to play a significant role in the microbial consortia. We present the complete genome sequence and feature analysis of Thermococcus onnurineus NA1 isolated from a deep-sea hydrothermal vent area, which reveal clues to its physiology. Based on results of genomic analysis, T. onnurineus NA1 possesses the metabolic pathways for organotrophic growth on peptides, amino acids, or sugars. More interesting was the discovery that the genome encoded unique proteins that are involved in carboxydotrophy to generate energy by oxidation of CO to CO2, thereby providing a mechanistic basis for growth with CO as a substrate. This lithotrophic feature in combination with carbon fixation via RuBisCO (ribulose 1,5-bisphosphate carboxylase/oxygenase) introduces a new strategy with a complementing energy supply for T. onnurineus NA1 potentially allowing it to cope with nutrient stress in the surrounding of hydrothermal vents, providing the first genomic evidence for the carboxydotrophy in Thermococcus.

Original languageEnglish
Pages (from-to)7491-7499
Number of pages9
JournalJournal of Bacteriology
Volume190
Issue number22
DOIs
StatePublished - Nov 2008

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