The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis

Jeong Yong Moon; Ashik Mosaddik; Hana Kim; Moonjae Cho; Hyung Kyoon Choi; Young Suk Kim; Somi Kim Cho

doi:10.1016/j.foodchem.2010.09.007

The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis

Jeong Yong Moon, Ashik Mosaddik, Hana Kim, Moonjae Cho, Hyung Kyoon Choi, Young Suk Kim, Somi Kim Cho

Department of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 μg/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 μg/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3′, 4′, 5′ trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer.

Original language	English
Pages (from-to)	369-375
Number of pages	7
Journal	Food Chemistry
Volume	125
Issue number	2
DOIs	https://doi.org/10.1016/j.foodchem.2010.09.007
State	Published - 15 Mar 2011

Bibliographical note

Funding Information:
This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Koran Government (MEST) (No. R01-2009-000-20492-0 ) and Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2009-0094061 ).

Keywords

Apoptosis
Caspase-3
Guava
High performance liquid chromatography coupled with photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS)
Human gastric carcinoma cell
Phytochemical
SNU-16

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10.1016/j.foodchem.2010.09.007

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@article{20064ca37a814cc1af97b3970e9de21b,

title = "The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis",

abstract = "The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 μg/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 μg/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3′, 4′, 5′ trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer.",

keywords = "Apoptosis, Caspase-3, Guava, High performance liquid chromatography coupled with photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS), Human gastric carcinoma cell, Phytochemical, SNU-16",

author = "Moon, {Jeong Yong} and Ashik Mosaddik and Hana Kim and Moonjae Cho and Choi, {Hyung Kyoon} and Kim, {Young Suk} and Cho, {Somi Kim}",

note = "Funding Information: This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Koran Government (MEST) (No. R01-2009-000-20492-0 ) and Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2009-0094061 ).",

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doi = "10.1016/j.foodchem.2010.09.007",

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T1 - The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis

AU - Moon, Jeong Yong

AU - Mosaddik, Ashik

AU - Kim, Hana

AU - Cho, Moonjae

AU - Choi, Hyung Kyoon

AU - Kim, Young Suk

AU - Cho, Somi Kim

N1 - Funding Information: This study was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Koran Government (MEST) (No. R01-2009-000-20492-0 ) and Priority Research Centers Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2009-0094061 ).

PY - 2011/3/15

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N2 - The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 μg/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 μg/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3′, 4′, 5′ trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer.

AB - The antiproliferative activities of the chloroform fraction (CF) of guava (Psidium cattleianum Sabine) leaf extract were evaluated using several cancer cell lines. Maximum cytotoxicity was observed in SNU-16, a human gastric carcinoma cell line, at concentrations of 50-100 μg/ml. Flow cytometric analysis demonstrated that CF treatment resulted in a marked accumulation of SNU-16 cells in the sub-G1 phase at concentrations of 100-200 μg/ml. The induction of apoptosis in SNU-16 cells was confirmed by immunoblotting using antibodies against Bcl-2, Bax, poly (ADP-ribose) polymerase (PARP), caspase-8, and caspase-3. The major CF phytochemicals were identified as ferulic acid, genistein, 3′, 4′, 5′ trimethoxy flavone, phlorizin, and oleanolic acid by high performance liquid chromatography coupled with a photo diode array and electrospray ionisation mass spectrometry (HPLC-PDA-ESI-MS). The results suggest that phytochemicals in the CF of guava (P. cattleianum) leaf extract induce apoptosis in SNU-16 cells. These findings may lead to new strategies for treating human gastric cancer.

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JF - Food Chemistry

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ER -

The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis

Abstract

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Keywords

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