Abstract
The birthdates of GABAergic amacrine cells in the rat retina were investigated by immunocytochemistry using anti-GABA and anti-bromodeoxyuridine (BrdU) antisera. The ratio of co-localization of GABA to BrdU increased gradually from embryonic-day 13 (E13) and showed a peak value on E18 in the central retina and on E20 in the periphery. After birth, until postnatal-day 3 (P3), a few co-localized cells were observed in the inner nuclear layer (INL). However, in the peripheral retina, co-localized cells were observed in the INL and ganglion cell layer until P5. Our results suggest that the birthdates of GABA-immunoreactive cells vary, depending on cell-type and that there is a temporal lag in the GABA-immunoreactive cell production in the peripheral retina relative to the central retina.
| Original language | English |
|---|---|
| Pages (from-to) | 309-314 |
| Number of pages | 6 |
| Journal | Experimental Brain Research |
| Volume | 128 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1999 |
Bibliographical note
Funding Information:Acknowledgements We are indebted to Dr. E. Fletcher at the Department of Anatomy and Cell Biology, The University of Melbourne in Australia, for her helpful comments and improving the English. This work was supported by a 1997 Basic Research Grant of Ministry of Education in Korea and by a Biotec grant (1998). We are grateful to Hee-Duck Rho and Byoung-Ouk Hong for their technical assistance.
Keywords
- Bromodeoxyuridine
- GABA
- Immunohistochemistry
- Proliferation
- Rat
- Retina