The axon guidance defect of the telencephalic commissures of the JSAP1-deficient brain was partially rescued by the transgenic expression of JIP1

Hye Yeong Ha, Ik Hyun Cho, Kang Woo Lee, Ko Woon Lee, Ji Young Song, Kyoung Shim Kim, Young Mi Yu, Ja Kyeong Lee, Jin Sook Song, Sung Don Yang, Hee Sup Shin, Pyung Lim Han

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The JNK interacting protein, JSAP1, has been identified as a scaffold protein for mitogen-activated protein kinase (MAPK) signaling pathways and as a linker protein for the cargo transport along the axons. To investigate the physiological function of JSAP1 in vivo, we generated mice lacking JSAP1. The JSAP1 null mutation produced various developmental deficits in the brain, including an axon guidance defect of the corpus callosum, in which phospho-FAK and phospho-JNK were distributed at reduced levels. The axon guidance defect of the corpus callosum in the jsap1-/- brain was correlated with the misplacement of glial sling cells, which reverted to their normal position after the transgenic expression of JNK interacting protein 1(JIP1). The transgenic JIP1 partially rescued the axon guidance defect of the corpus callosum and the anterior commissure of the jsap1-/- brain. The JSAP1 null mutation impaired the normal distribution of the Ca+2 regulating protein, calretinin, but not the synaptic vesicle marker, SNAP-25, along the axons of the thalamocortical tract. These results suggest that JSAP1 is required for the axon guidance of the telencephalic commissures and the distribution of cellular protein(s) along axons in vivo, and that the signaling network organized commonly by JIP1 and JSAP1 regulates the axon guidance in the developing brain.

Original languageEnglish
Pages (from-to)184-199
Number of pages16
JournalDevelopmental Biology
Volume277
Issue number1
DOIs
StatePublished - 1 Jan 2005

Keywords

  • Anterior commissures
  • Corpus callosum
  • JIP1
  • JNK
  • JSAP1
  • Scaffold protein

Fingerprint

Dive into the research topics of 'The axon guidance defect of the telencephalic commissures of the JSAP1-deficient brain was partially rescued by the transgenic expression of JIP1'. Together they form a unique fingerprint.

Cite this