Temporal and spatial differentiation in microhabitat use: Implications for reproductive isolation and ecological niche specification

Amaël Borzée, Jun Young Kim, Marina Andrade Martins DA Cunha, Donggeun Lee, Eunchong Sin, Sunmin Oh, Yoonjung Yi, Yikweon Jang

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


Niche differentiation enables ecologically similar species to coexist by lessening competition over food and/or shelters and may be critical for reproductive isolation between closely related species in close proximity. Because no extra traits need to evolve, spatial and temporal differentiation may readily take place to complement other isolating mechanisms. Two closely related treefrog species occur together in Korea: the endangered Hyla suweonensis and the widespread Hyla japonica. Advertisement calls are differentiated, but it is unclear whether call difference is sufficient for reproductive isolation. We tracked individuals of both species to study fine-scale differentiation in microhabitat use in the diel cycle of the breeding season using a harmonic direction finder. tracking male movement patterns of both species revealed spatial and temporal differentiation in microhabitat use for calling and resting during the breeding season. Males of both H. suweonensis and H. japonica occurred in all 5 microhabitats identified in this study: rice paddy, ground, buried, grass and bush. Both treefrog species showed general similarities in calling from rice paddies and resting in grass and bush. However, H. suweonensis moved into rice paddies and produced advertisement calls 3 h earlier than H. japonica. These differences likely minimize contact between the species and provide an additional isolating mechanism. In addition, the activity of H. suweonensis may be contributing to the decline of this species, as resting in grass would increase dangers from predatory birds and habitat disturbance.

Original languageEnglish
Pages (from-to)375-387
Number of pages13
JournalIntegrative zoology
Issue number5
StatePublished - 1 Sep 2016

Bibliographical note

Publisher Copyright:
© 2016 International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.


  • harmonic direction finding
  • hylid treefrog
  • pre-mating isolation mechanisms
  • spatial isolation
  • temporal isolation


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